NF-kB p65 ELISA 试剂盒
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Quick Overview for NF-kB p65 ELISA 试剂盒 (ABIN8126254)
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See all NF-kB p65 (NFkBP65) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.313 ng/mL
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原理
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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灵敏度
- 0.188 ng/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Human NF-kB p65 concentrations in serum, plasma. It also detects cytoplasmic, nuclear and total NF-kB p65 from cells. Detection Range: 0.313 - 20 ng/mL Sensitivity: < 0.188 ng/mL
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Nuclear Factor-kB p65 (NFkBP65) ELISA Kit
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Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric DNA-Binding ELISA
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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板类型
- Pre-coated
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实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human p65 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human p65 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human p65 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human p65 can be calculated using the standard curve. Kit Components
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限制
- 仅限研究用
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储存方法
- Please refer to the manual
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- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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别名
- NF-kB p65 (Nuclear Factor Kappa B p65)
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途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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