NF-kB p65 ELISA 试剂盒
(1 reference)-
- 抗原 See all NF-kB p65 (NFkBP65) ELISA试剂盒
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- NF-kB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
- Analytical Method
- Quantitative
- 产品特性
- A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p65) is detected by addition of a specific primary antibody directed against NF-kB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. NF-kB (p65) Transcription Factor Assay detects human NF-kB (p65). It will not cross-react with NF-kB (p50).
- Featured
- Discover our best selling NFkBP65 ELISA Kit
-
Nuclear Factor-kB p65 (NFkBP65) ELISA Kit
NFkBP65 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人, 大鼠, 小鼠 pSer536 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
-
- 应用备注
- NF-kB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p65) is detected by addition of a specific primary antibody directed against NF-kB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. NF-kB (p65) Transcription Factor Assay detects human NF-kB (p65). It will not cross-react with NF-kB (p50).
- 说明
-
Detection Kit Type: ELISA Kit
- 板类型
- Pre-coated
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C/-20 °C
-
-
: "Methylglyoxal Acts as a Tumor-Promoting Factor in Anaplastic Thyroid Cancer." in: Cells, Vol. 8, Issue 6, (2019) (PubMed).
-
: "Methylglyoxal Acts as a Tumor-Promoting Factor in Anaplastic Thyroid Cancer." in: Cells, Vol. 8, Issue 6, (2019) (PubMed).
-
- 抗原 See all NF-kB p65 (NFkBP65) ELISA试剂盒
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- 别名
- NF kB (p65) (NFkBP65 产品)
- 别名
- C-Rel ELISA Kit, NFKB3 ELISA Kit, p65 ELISA Kit, NFkB ELISA Kit, c-rel ELISA Kit, zgc:100833 ELISA Kit, Xrel2 ELISA Kit, Xrel3 ELISA Kit, rel ELISA Kit, rel-A ELISA Kit, rel2 ELISA Kit, rel3 ELISA Kit, v-rel ELISA Kit, xrel ELISA Kit, REL proto-oncogene, NF-kB subunit ELISA Kit, RELA proto-oncogene, NF-kB subunit ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit, v-rel avian reticuloendotheliosis viral oncogene homolog ELISA Kit, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog ELISA Kit, REL ELISA Kit, RELA ELISA Kit, Rela ELISA Kit, Rel ELISA Kit, rel ELISA Kit, rel.L ELISA Kit
- 背景
-
The NF-kB/Rel family of transcription factors is comprised of several structurally related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), c-Rel/NF-kB [1]. Members of this family are responsible for regulating over 150 target genes, including the expression of inflammatory cytokines, chemokines, immunoreceptors and cell adhesion molecules. Because of this, NF-kB has often been called a ‘central mediator of the human immune response' [2]. Acting as dimers, these transcription factors bind to DNA sequences, collectively called kB, sites thereby regulating expression of target genes. In most cells, Rel/ NF-kB transcription complexes are present in an inactive form in the cytoplasm, bound to an inhibitor IB. Certain stimuli result in the phosphorylation, ubiquitination and subsequent degradation of IkB proteins thereby enabling translocation of NF-kB into the nucleus [3]. The most common Rel/NF-kB dimmer in mammals contains p50-RelA (p50/p65) heterodimers and is specifically called NF-kB. One of the target genes activated by NF-kB is that encoding IkBα. This feedback mechanism allows newly-synthesized IkBα to enter the nucleus, remove NF-kB from DNA and transport it back to the cytoplasm thereby restoring its inactive state. The importance of Rel/NF-kB transcription factors in human inflammation and certain diseases makes them attractive targets for potential therapeutics [4-6].
Synonyms: NF-kB Transcription Factor Kit, p65 kit, EIA kit, NF-κB (p65) Transcription Factor Assay - 途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
-
