NF-kB p65 ELISA 试剂盒
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- 抗原 See all NF-kB p65 (NFkBP65) ELISA试剂盒
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- 抗原表位
- pSer536, total
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适用
- 人, 大鼠, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human/Mouse/Rat Phospho-NF-KB p65 (S536) ELISA and Total NF-KB p65 Kit. This assay semi-quantitatively measures phophorylated NF-KB p65 (Ser536) and Total NF-KB p65 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human NF-KB p65 phosphorylated at site Serine-536 and Total NF-KB p65.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Nuclear Factor-kB p65 (NFkBP65) ELISA Kit
NFkBP65 适用: 人 Colorimetric DNA-Binding ELISA
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all NF-kB p65 (NFkBP65) ELISA试剂盒
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- 别名
- NFKBP65 (NFkBP65 产品)
- 别名
- C-Rel ELISA Kit, NFKB3 ELISA Kit, p65 ELISA Kit, NFkB ELISA Kit, c-rel ELISA Kit, zgc:100833 ELISA Kit, Xrel2 ELISA Kit, Xrel3 ELISA Kit, rel ELISA Kit, rel-A ELISA Kit, rel2 ELISA Kit, rel3 ELISA Kit, v-rel ELISA Kit, xrel ELISA Kit, REL proto-oncogene, NF-kB subunit ELISA Kit, RELA proto-oncogene, NF-kB subunit ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit, v-rel avian reticuloendotheliosis viral oncogene homolog ELISA Kit, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog ELISA Kit, REL ELISA Kit, RELA ELISA Kit, Rela ELISA Kit, Rel ELISA Kit, rel ELISA Kit, rel.L ELISA Kit
- 背景
- Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-KB p65) phosphorylated at Serine-536 and total NF-KB p65.
- 基因ID
- 5970
- UniProt
- Q04206
- 途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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