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NF-kB p65 IQ-ELISA 试剂盒

NFkBP65 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN6386008
发货至: 中国
  • 抗原 See all NF-kB p65 (NFkBP65) IQ-ELISA Kits
    NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
    适用
    • 27
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    检测方法
    qPCR
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Immunoquantitative (PCR-Based) RelA (NFkB p65 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Semi-Quantitative
    产品特性
    IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
    组件
    • RelA (NFkB p65 Microplate (Item A): 96 well PCR plate coated with anti-Human RelA (NFkB p65
    • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
    • Standards (Item C): 2 vials of recombinant Human RelA (NFkB p65
    • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for RelA (NFkB p65 (Item F): 2 vials of a 4x concentrated solution of anti-Human RelA (NFkB p65 affinity reagent
    • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
    • Primer Solution (Item I): 1.7 ml vial
    • PCR Master Mix (Item J): 1.2 ml vial
    • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
    • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
    试剂未包括
    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80°C
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  • 应用备注
    The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
    说明

    The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

    样本量
    25 µL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
    5. Add 15μL Primer solution 10μL of PCR master mix to each well
    6. Run real-time PCR
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C,-80 °C
    储存方法
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all NF-kB p65 (NFkBP65) IQ-ELISA Kits
    NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
    别名
    RelA (NFkB p65) (NFkBP65 产品)
    别名
    C-Rel IQ-ELISA Kit, NFKB3 IQ-ELISA Kit, p65 IQ-ELISA Kit, NFkB IQ-ELISA Kit, c-rel IQ-ELISA Kit, zgc:100833 IQ-ELISA Kit, Xrel2 IQ-ELISA Kit, Xrel3 IQ-ELISA Kit, rel IQ-ELISA Kit, rel-A IQ-ELISA Kit, rel2 IQ-ELISA Kit, rel3 IQ-ELISA Kit, v-rel IQ-ELISA Kit, xrel IQ-ELISA Kit, REL proto-oncogene, NF-kB subunit IQ-ELISA Kit, RELA proto-oncogene, NF-kB subunit IQ-ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A (avian) IQ-ELISA Kit, v-rel avian reticuloendotheliosis viral oncogene homolog IQ-ELISA Kit, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog IQ-ELISA Kit, REL IQ-ELISA Kit, RELA IQ-ELISA Kit, Rela IQ-ELISA Kit, Rel IQ-ELISA Kit, rel IQ-ELISA Kit, rel.L IQ-ELISA Kit
    途径
    NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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