TNFRSF12A ELISA 试剂盒
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- 抗原 See all TNFRSF12A ELISA试剂盒
- TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 95-25000 pg/mL
- 最低检测浓度
- 95 pg/mL
- 应用范围
- ELISA
- 原理
- Mouse TWEAK R (TNFRSF12) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, TWEAK, VCAM-1, VEGF.
- 灵敏度
- 95 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product TNFRSF12A ELISA Kit
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Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) ELISA Kit
TNFRSF12A 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) ELISA KitTNFRSF12A 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) ELISA KitTNFRSF12A 适用: 大鼠 Colorimetric Sandwich ELISA 0.3-80 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples3 - 30 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all TNFRSF12A ELISA试剂盒
- TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
- 别名
- TWEAK R (TNFRSF12A 产品)
- 别名
- CD266 ELISA Kit, FN14 ELISA Kit, TWEAKR ELISA Kit, AI255180 ELISA Kit, C87282 ELISA Kit, Fn14 ELISA Kit, HPIP ELISA Kit, TWEAK-R ELISA Kit, TweakR ELISA Kit, TNF receptor superfamily member 12A ELISA Kit, tumor necrosis factor receptor superfamily, member 12a ELISA Kit, TNFRSF12A ELISA Kit, Tnfrsf12a ELISA Kit
- 背景
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Gene Names: Tnfrsf12a Fgfrp2 Fn14
Protein names: Tumor necrosis factor receptor superfamily member 12A (Fibroblast growth factor-inducible immediate-early response protein 14) (FGF-inducible 14) (Fibroblast growth factor-regulated protein 2) (Tweak-receptor) (TweakR) (CD antigen CD266)
- 基因ID
- 27279
- UniProt
- Q9CR75
- 途径
- Apoptosis, Regulation of Cell Size
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