TNFRSF12A ELISA 试剂盒
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- 抗原 See all TNFRSF12A ELISA试剂盒
- TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.3-80 ng/mL
- 最低检测浓度
- 0.3 ng/mL
- 应用范围
- ELISA
- 原理
- Rat TWEAK R (TNFRSF12) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB, Activin A, Adiponectin, B7-1, CD48, CTACK, Decorin, Eotaxin, EphA5, Erythropoietin, FGF-BP, Fit-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1, gp130, HGF, IL-1 R6, IL-1 R alpha, IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1, TIMP-2, TREM-1
- 灵敏度
- 0.3 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product TNFRSF12A ELISA Kit
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Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) ELISA Kit
TNFRSF12A 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) ELISA KitTNFRSF12A 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) ELISA KitTNFRSF12A 适用: 小鼠 Colorimetric Sandwich ELISA 95-25000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples4 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all TNFRSF12A ELISA试剂盒
- TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
- 别名
- TWEAK R (TNFRSF12A 产品)
- 别名
- CD266 ELISA Kit, FN14 ELISA Kit, TWEAKR ELISA Kit, AI255180 ELISA Kit, C87282 ELISA Kit, Fn14 ELISA Kit, HPIP ELISA Kit, TWEAK-R ELISA Kit, TweakR ELISA Kit, TNF receptor superfamily member 12A ELISA Kit, tumor necrosis factor receptor superfamily, member 12a ELISA Kit, TNFRSF12A ELISA Kit, Tnfrsf12a ELISA Kit
- 背景
- Tumor necrosis factor receptor superfamily, member 12a (Type 1 transmembrane protein FN14)
- 基因ID
- 302965
- UniProt
- Q80XX9
- 途径
- Apoptosis, Regulation of Cell Size
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