TNFRSF10A ELISA 试剂盒
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- 抗原 See all TNFRSF10A ELISA试剂盒
- TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 40-10.000 pg/mL
- 最低检测浓度
- 40 pg/mL
- 应用范围
- ELISA
- 原理
- Custom Human TRAIL Receptor 1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human TRAIL R1.
- 灵敏度
- 40 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A) ELISA Kit
TNFRSF10A 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A) ELISA KitTNFRSF10A 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all TNFRSF10A ELISA试剂盒
- TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
- 别名
- TRAIL R1 (TNFRSF10A 产品)
- 别名
- APO2 ELISA Kit, CD261 ELISA Kit, DR4 ELISA Kit, TRAILR-1 ELISA Kit, TRAILR1 ELISA Kit, TNFRSF10A ELISA Kit, TNF receptor superfamily member 10a ELISA Kit, tumor necrosis factor receptor superfamily member 26 ELISA Kit, tumor necrosis factor receptor superfamily, member 10a ELISA Kit, TNFRSF10A ELISA Kit, LOC510798 ELISA Kit
- 背景
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Gene Names: TNFSF10 APO2L TRAIL
Protein names: Tumor necrosis factor ligand superfamily member 10 (Apo-2 ligand) (Apo-2L) (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253)
- 基因ID
- 8797
- UniProt
- O00220
- 途径
- Apoptosis, Positive Regulation of Endopeptidase Activity
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