TNFSF14 ELISA 试剂盒
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- 抗原 See all TNFSF14 ELISA试剂盒
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15-6000 pg/mL
- 最低检测浓度
- 15 pg/mL
- 应用范围
- ELISA
- 原理
- Human LIGHT (TNFSF14) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 15 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling TNFSF14 ELISA Kit
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- Discover our top product TNFSF14 ELISA Kit
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Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA Kit
TNFSF14 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Saliva, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 人 AA 74-240 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Plasma, Serum, Urine
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 大鼠 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 小鼠 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all TNFSF14 ELISA试剂盒
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- 别名
- LIGHT (TNFSF14 产品)
- 别名
- TNFSF14 ELISA Kit, CD258 ELISA Kit, HVEML ELISA Kit, LIGHT ELISA Kit, LTg ELISA Kit, TR2 ELISA Kit, HVEM-L ELISA Kit, Ly113 ELISA Kit, TNF superfamily member 14 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 14 ELISA Kit, TNFSF14 ELISA Kit, Tnfsf14 ELISA Kit
- 背景
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Gene Names: TNFSF14 HVEML LIGHT UNQ391/PRO726
Protein names: Tumor necrosis factor ligand superfamily member 14 (Herpes virus entry mediator ligand) (HVEM-L) (Herpesvirus entry mediator ligand) (CD antigen CD258) [Cleaved into: Tumor necrosis factor ligand superfamily member 14, membrane form, Tumor necrosis factor ligand superfamily member 14, soluble form]
- 基因ID
- 8740
- UniProt
- O43557
- 途径
- Cancer Immune Checkpoints
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