TNFSF14 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for TNFSF14 ELISA 试剂盒 (ABIN1672886)
抗原
See all TNFSF14 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 31.2 pg/mL
-
原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFSF14/LIGHT
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NSO
Immunogen sequence: D74-V240 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- <10pg/mL
-
试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
-
免疫原
-
Expression system for standard: NSO
Immunogen sequence: D74-V240
-
-
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA Kit
TNFSF14 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Saliva, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 人 Colorimetric Sandwich ELISA 63-4000 pg/mL Cell Lysate, Plasma, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Plasma, Serum, Urine
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 大鼠 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 小鼠 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
说明
-
Tissue Specificity: Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
-
板类型
- Pre-coated
-
实验流程
- human TNFSF14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF14 has been precoated onto 96-well plates. Standards(NSO, D74-V240) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFSF14 amount of sample captured in plate.
-
实验流程
-
Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TNFSF14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFSF14 standard solution and each sample be measured in duplicate.
-
实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 323, Standard deviation: 13.6, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 853, Standard deviation: 43.5, CV(%): 5.1
- Sample 3: n=16, Mean(pg/ml): 1285, Standard deviation: 46.3, CV(%): 3.6,
- Sample 1: n=24, Mean(pg/ml): 354, Standard deviation: 27.6, CV(%): 7.8
- Sample 2: n=24, Mean(pg/ml): 887, Standard deviation: 67.4, CV(%): 7.6
- Sample 3: n=24, Mean(pg/ml): 1366, Standard deviation: 97, CV(%): 7.1
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
-
有效期
- 12 months
-
-
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
-
别名
- TNFSF14
-
背景
-
Protein Function: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.
Background: Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor(TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
Synonyms: Tumor necrosis factor ligand superfamily member 14,Herpes virus entry mediator ligand,HVEM-L,Herpesvirus entry mediator ligand,CD258,Tumor necrosis factor ligand superfamily member 14, membrane form,Tumor necrosis factor ligand superfamily member 14, soluble form,TNFSF14,HVEML, LIGHT,UNQ391/PRO726,
Full Gene Name: Tumor necrosis factor ligand superfamily member 14
Cellular Localisation: Tumor necrosis factor ligand superfamily member 14, membrane form: Cell membrane, Single-pass type II membrane protein. -
基因ID
- 8740
-
UniProt
- O43557
-
途径
- Cancer Immune Checkpoints
抗原 See all TNFSF14 ELISA试剂盒
-
