NFKBIA ELISA 试剂盒
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- 抗原 See all NFKBIA ELISA试剂盒
- NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human IkB-alpha/NF-kappa-BIA ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human IkB-alpha.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product NFKBIA ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all NFKBIA ELISA试剂盒
- NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
- 别名
- IkB-alpha (NF-kappa-BIA) (NFKBIA 产品)
- 别名
- IKBA ELISA Kit, MAD-3 ELISA Kit, NFKBI ELISA Kit, AI462015 ELISA Kit, Nfkbi ELISA Kit, RL/IF-1 ELISA Kit, ECI-6/IKBA ELISA Kit, NFKBIA ELISA Kit, ikbaB ELISA Kit, wu:fb81f08 ELISA Kit, zgc:56666 ELISA Kit, zgc:77616 ELISA Kit, ikbaA ELISA Kit, wu:fj16a07 ELISA Kit, zgc:85769 ELISA Kit, NFKB inhibitor alpha ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a ELISA Kit, NFKBIA ELISA Kit, Nfkbia ELISA Kit, nfkbiab ELISA Kit, nfkbiaa ELISA Kit
- 基因ID
- 4792
- UniProt
- P25963
- 途径
- NF-kappaB Signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling
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