IL-1 beta ELISA 试剂盒
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Quick Overview for IL-1 beta ELISA 试剂盒 (ABIN577090)
抗原
See all IL-1 beta (IL1B) ELISA试剂盒适用
检测方法
实验类型
应用范围
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原理
- This IL-1 enzyme linked immunosorbent assay (ELISA) applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-1 . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-1 and incubated. IL-1 if present, will bind and become immobilized by the antibody pre-coated on the wells and then become
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Analytical Method
- Quantitative
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灵敏度
- The minimum detectable quantities of human IL-1 as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 2.0 pg/mL and 2.0 pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities
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组件
- Standards: 1 set/2 vials
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Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 AA 117-269 Colorimetric Sandwich ELISA 3.9-250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 AA 117-268 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鼠 AA 118-269 Colorimetric Sandwich ELISA 12.5-800 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 鱼 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鸡 Colorimetric Competition ELISA 0.27 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Without preservative
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- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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别名
- Interleukin-1 beta (IL-1beta)
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背景
- Interleukin-16 (IL-16) discovered in 19821, known formerly as lymphocyte chemoattractant factor (LCF) is a lymphocyte chemoattractant factor of T cell origin with selective activity for CD4+ T cells. LCF was designated interleukin-16 (IL-16) in 1995. IL-16 is a product of CD8+ cells, CD4+ cells2, eosinophils3, mast cells and epithelial cells derived from asthmatics4. Expression of IL-16 in asthmatic epithelium correlates with the number of infiltrating CD4+ T cells5. LCF was originally identified and purified from the supernatants of ConA-stimulated peripheral blood mononuclear cells (PBMC). In addition to its chemotactic activity, it is a competence growth factor selective for CD4+ T cells dependent on an interaction with CD4 for induction of functional activity. Its cDNA codes for a novel 14-kDa protein into a homotetrameric form is required for induction of biologic activity. IL-16 appears in culture supernatants as a relative molecular mass (Mr) ~56, biologically active, non-covalently linked tetramer, but migrates in monomeric form in SDS PAGE. Eluted monomeric peptides are inactive but reaggregate to Mr 56, regaining biological activity6. The protein expressed from the IL-16 cDNA demonstrates all the functions and chemical features of the native protein, including an identical pI, and autoaggregation into functional tetramers. IL-16 is performed and stored in biologically active form in CD8+. T cells from which it is secreted following stimulation with histamine via H2 type receptors. The secretory process occurs within 4 hrs and does not require transcription, translation, or new protein synthesis. IL-16's ability to inhibit the MLR and other antigen induced activation suggests that it may be useful in inhibiting allograft rejection. Because IL-16 selectively induces IL-2 responsiveness in CD4+ T cells it may be useful (along with IL-2) for selective CD4+ T cell immune reconstitution in individuals with lymphopenia, for example following chemotherapy or HIV-1 infection. In the latter circumstance, its ability to inhibit HIV transcription should provide a protective role if used as a therapy in HIV-1 infected individuals7. Inhibitors of IL-16 chemotactic function may be useful in diseases in which it appears to play a prominent role in the inflammatory process. The presence of IL-16 early after antigen challenge in asthma8, the marked upregulation of IL-16 synthesis by epithelium of asthmatics along with the correlation of IL-16 protein with the number of infiltrating CD4+ S7.5(2) IL-16 2 _x000C_ T cells in the airways of human asthmatics suggest that therapeutics aims at blocking IL- 16 synthesis or function may be valuable in this disease. This IL-16 ELISA is a 4.5 hour solid phase immunoassay readily applicable to measure IL-16 in serum, plasma, cell culture supernatant, and other biological fluids in the range of to 32 pg/mL. It showed no cross-reactivity with other cytokines tested such as EGF, EPO, GM-CSF, IL-1 , IL-7, IL-8, IFN- , MCAF, MCP-3, M-CSF, SAA, TGF- , and TNF-
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途径
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
抗原 See all IL-1 beta (IL1B) ELISA试剂盒
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