TRAIL ELISA 试剂盒
-
- 抗原 See all TRAIL (TNFSF10) ELISA试剂盒
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). No significant cross-reactivity or interference between Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) and analogues was observed.
- 灵敏度
- 6.2 pg/mL
- Featured
- Discover our best selling TNFSF10 ELISA Kit
- Top Product
- Discover our top product TNFSF10 ELISA Kit
-
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA Kit
TNFSF10 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 猴 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Serum, Urine
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitWide Range TNFSF10 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 人 AA 95-281 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all TRAIL (TNFSF10) ELISA试剂盒
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- 别名
- Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TNFSF10 产品)
- 别名
- APO2L ELISA Kit, Apo-2L ELISA Kit, CD253 ELISA Kit, TL2 ELISA Kit, TRAIL ELISA Kit, A330042I21Rik ELISA Kit, AI448571 ELISA Kit, APO-2L ELISA Kit, Ly81 ELISA Kit, Trail ELISA Kit, tl2 ELISA Kit, apo2l ELISA Kit, cd253 ELISA Kit, trail ELISA Kit, apo-2l ELISA Kit, trail1 ELISA Kit, xtrail1 ELISA Kit, tnfsf10l2 ELISA Kit, zgc:92320 ELISA Kit, TNLG6A ELISA Kit, TNF superfamily member 10 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 10 ELISA Kit, tumor necrosis factor superfamily member 10 ELISA Kit, TNF superfamily member 10 S homeolog ELISA Kit, TNFSF10 ELISA Kit, Tnfsf10 ELISA Kit, tnfsf10.S ELISA Kit, tnfsf10 ELISA Kit
- 背景
-
Gene Name: Tumor Necrosis Factor Related Apoptosis Inducing Ligand
Gene Aliases: CD253, TNFSF10, APO2L, Apo2-L, TL2, TRAIL, Tumor Necrosis Factor Ligand Superfamily Member 10, Apo-2 Ligand
- 基因ID
- 22035
- UniProt
- P50592
- 途径
- Apoptosis, Positive Regulation of Endopeptidase Activity
-
