TNFSF14 ELISA 试剂盒
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- 抗原 See all TNFSF14 ELISA试剂盒
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14). No significant cross-reactivity or interference between Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) and analogues was observed.
- 灵敏度
- 6.5 pg/mL
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- Discover our best selling TNFSF14 ELISA Kit
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Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA Kit
TNFSF14 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Saliva, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 人 AA 74-240 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Plasma, Serum, Urine
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 大鼠 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) ELISA KitTNFSF14 适用: 小鼠 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all TNFSF14 ELISA试剂盒
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- 别名
- Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14 产品)
- 别名
- TNFSF14 ELISA Kit, CD258 ELISA Kit, HVEML ELISA Kit, LIGHT ELISA Kit, LTg ELISA Kit, TR2 ELISA Kit, HVEM-L ELISA Kit, Ly113 ELISA Kit, TNF superfamily member 14 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 14 ELISA Kit, TNFSF14 ELISA Kit, Tnfsf14 ELISA Kit
- 背景
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Gene Name: Tumor Necrosis Factor Ligand Superfamily, Member 14
Gene Aliases: CD258, TR2, HVEML, LIGHT, LTg, Herpes virus entry mediator ligand, Herpesvirus entry mediator ligand
- 基因ID
- 8740
- UniProt
- O43557
- 途径
- Cancer Immune Checkpoints
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