HFE2 ELISA 试剂盒
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- 抗原 See all HFE2 ELISA试剂盒
- HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 12.35 ng/mL - 1000 ng/mL
- 最低检测浓度
- 12.35 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Hemojuvelin (HJV). No significant cross-reactivity or interference between Hemojuvelin (HJV) and analogues was observed.
- 灵敏度
- 4.93 ng/mL
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Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA Kit
HFE2 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 人 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 大鼠 AA 36-396 Colorimetric Sandwich ELISA
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 人 AA 36-400 Colorimetric Sandwich ELISA
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 小鼠 AA 33-393 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemojuvelin (HJV) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemojuvelin (HJV) and unlabeled Hemojuvelin (HJV) (Standards or samples) with the pre-coated antibody specific to Hemojuvelin (HJV). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemojuvelin (HJV) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemojuvelin (HJV) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemojuvelin (HJV) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemojuvelin (HJV) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all HFE2 ELISA试剂盒
- HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))
- 别名
- Hemojuvelin (HFE2 产品)
- 别名
- HFE2A ELISA Kit, HJV ELISA Kit, JH ELISA Kit, RGMC ELISA Kit, HFE2 ELISA Kit, DKFZp468F2322 ELISA Kit, 2310035L15Rik ELISA Kit, 5230400G09Rik ELISA Kit, AI414844 ELISA Kit, AI789733 ELISA Kit, DL-M ELISA Kit, Rgmc ELISA Kit, hemojuvelin ELISA Kit, RGMr ELISA Kit, hjv ELISA Kit, id:ibd3464 ELISA Kit, wu:fb38f10 ELISA Kit, zgc:136698 ELISA Kit, hemochromatosis type 2 (juvenile) ELISA Kit, hemochromatosis type 2 ELISA Kit, HFE2 ELISA Kit, Hfe2 ELISA Kit, hfe2 ELISA Kit
- 背景
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Gene Name: Hemojuvelin
Gene Aliases: HFE2, HFE2A, HJV, JH, RGMC, hemochromatosis type 2(Juvenile), Haemojuvelin, RGM domain family member C
- 基因ID
- 148738
- UniProt
- Q6ZVN8
- 途径
- Transition Metal Ion Homeostasis
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