HFE2 ELISA 试剂盒
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- 抗原 See all HFE2 ELISA试剂盒
- HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))
- 抗原表位
- AA 33-393
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Hemojuvelin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: Q33-D393 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: Q33-D393 - Featured
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Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA Kit
HFE2 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 人 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 大鼠 AA 36-396 Colorimetric Sandwich ELISA
Hemochromatosis Type 2 (Juvenile) (HFE2) ELISA KitHFE2 适用: 人 AA 36-400 Colorimetric Sandwich ELISA
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Muscle cell lineage. .
- 板类型
- Pre-coated
- 实验流程
- mouse Hemojuvelin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Hemojuvelin has been precoated onto 96-well plates. Standards(NSO, Q33-D393) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Hemojuvelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Hemojuvelin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Hemojuvelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Hemojuvelin standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.43, Standard deviation: 0.073, CV(%): 5.1
- Sample 2: n=16, Mean(ng/ml): 3.06, Standard deviation: 0.113, CV(%): 3.7
- Sample 3: n=16, Mean(ng/ml): 5.72, Standard deviation: 0.223, CV(%): 3.9,
- Sample 1: n=24, Mean(ng/ml): 1.46, Standard deviation: 0.11, CV(%): 7.5
- Sample 2: n=24, Mean(ng/ml): 3.24, Standard deviation: 0.237, CV(%): 7.3
- Sample 3: n=24, Mean(ng/ml): 5.91, Standard deviation: 0.402, CV(%): 6.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all HFE2 ELISA试剂盒
- HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))
- 别名
- HFE2 (HFE2 产品)
- 别名
- HFE2A ELISA Kit, HJV ELISA Kit, JH ELISA Kit, RGMC ELISA Kit, HFE2 ELISA Kit, DKFZp468F2322 ELISA Kit, 2310035L15Rik ELISA Kit, 5230400G09Rik ELISA Kit, AI414844 ELISA Kit, AI789733 ELISA Kit, DL-M ELISA Kit, Rgmc ELISA Kit, hemojuvelin ELISA Kit, RGMr ELISA Kit, hjv ELISA Kit, id:ibd3464 ELISA Kit, wu:fb38f10 ELISA Kit, zgc:136698 ELISA Kit, hemochromatosis type 2 (juvenile) ELISA Kit, hemochromatosis type 2 ELISA Kit, HFE2 ELISA Kit, Hfe2 ELISA Kit, hfe2 ELISA Kit
- 背景
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Protein Function: Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis. .
Background: Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of a three gene family (in vertebrates) called the repulsive guidance molecules. In mouse, the Hemojuvelin protein is encoded by the HFE2 gene, and it is mapped to Chromosome 3. It was concluded that downregulation of hepatic Hemojuvelin during inflammation may induce a temporary elimination of iron sensing. Hemojuvelin is a coreceptor for bone morphogenetic proteins (BMPs), and inhibition of endogenous BMP signaling reduces hepcidin expression and increases serum iron in mice. Furthermore, Hemojuvelin may play inhibitory roles in prostate cancer by suppressing cell growth, adhesion, migration and invasion.
Synonyms: Hemojuvelin,Hemochromatosis type 2 protein homolog,RGM domain family member C,Hfe2,Rgmc,
Full Gene Name: Hemojuvelin
Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor . Also released in the extracellular space.. - 基因ID
- 69585
- UniProt
- Q7TQ32
- 途径
- Transition Metal Ion Homeostasis
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