IL-1 beta ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for IL-1 beta ELISA 试剂盒 (ABIN5026930)
抗原
See all IL-1 beta (IL1B) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 7.8 pg/mL
-
原理
- This assay employs an antibody specific for anti-human IL-1b coated on a 96-well plate.
-
Analytical Method
- Quantitative
-
灵敏度
- 4.69 pg/mL
-
产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-1Beta concentrations in serum, plasma. Detection Range: 7.8 - 500 pg/mL Sensitivity: < 4.69 pg/mL
-
组件
- plate, standard, diluent, antibodies
-
试剂未包括
- pipettes, tubes, reader, incubator
-
-
Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 AA 117-269 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 AA 117-268 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鼠 AA 118-269 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 鱼 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鸡 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
样本量
- 100 μL
-
实验时间
- 3 - 4 h
-
板类型
- Pre-coated
-
实验流程
- This assay employs an antibody specific for anti-human IL-1b coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1b present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-1Beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1b bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
限制
- 仅限研究用
-
-
-
注意事项
- protect your eyes
-
储存条件
- 4 °C
-
储存方法
- Please refer to the Manual
-
-
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
-
别名
- IL-1 Beta (Interleukin 1 Beta)
-
途径
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
抗原 See all IL-1 beta (IL1B) ELISA试剂盒
-
