NF-kB p65 ELISA 试剂盒
Quick Overview for NF-kB p65 ELISA 试剂盒 (ABIN4889784)
抗原
See all NF-kB p65 (NFkBP65) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- Human NF-κB p65 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical NF-κB binding sequences to semi-quantitatively detect active NF-κB in lysates or nuclear extracts. Only available in North America.
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Analytical Method
- Semi-Quantitative
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特异性
- The olionucleotide/antibody pair provided in this kit recognizes human NF-κB p65 in whole lysates and nuclear extracts.
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产品特性
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- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96 well plate format)
- Assay can be completed within 5 hours
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组件
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- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions Absorbent paper
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Nuclear Factor-kB p65 (NFkBP65) ELISA Kit
NFkBP65 适用: 人 Colorimetric DNA-Binding ELISA
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人, 大鼠, 小鼠 pSer536 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared HRP-secondary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
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有效期
- 6 months
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- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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别名
- NFKBP65
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基因ID
- 5970
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UniProt
- Q04206
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途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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