PCNA ELISA 试剂盒
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- 抗原 See all PCNA ELISA试剂盒
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 2.5-600 ng/mL
- 最低检测浓度
- 2.5 ng/mL
- 应用范围
- ELISA
- 原理
- Human PCNA ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human PCNA
- 灵敏度
- 2.46 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling PCNA ELISA Kit
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- Discover our top product PCNA ELISA Kit
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Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit
PCNA 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Proliferating Cell Nuclear Antigen (PCNA) ELISA KitPCNA 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Proliferating Cell Nuclear Antigen (PCNA) ELISA KitPCNA 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Proliferating Cell Nuclear Antigen (PCNA) ELISA KitPCNA 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Proliferating Cell Nuclear Antigen (PCNA) ELISA KitPCNA 适用: 兔 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all PCNA ELISA试剂盒
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- 别名
- PCNA (PCNA 产品)
- 别名
- PCNAR ELISA Kit, Pcna/cyclin ELISA Kit, pcna ELISA Kit, AO090003000846 ELISA Kit, 53/13 ELISA Kit, CG9193 ELISA Kit, DmPCNA ELISA Kit, Dmel\\CG9193 ELISA Kit, MUS209 ELISA Kit, Mus209 ELISA Kit, PCNA ELISA Kit, PCNA/mus209 ELISA Kit, Pcna ELISA Kit, dPCNA ELISA Kit, l(2)02448 ELISA Kit, l(2)56F ELISA Kit, l(2)56Fa ELISA Kit, l(2)s1534 ELISA Kit, cb16 ELISA Kit, fb36g03 ELISA Kit, wu:fa28e03 ELISA Kit, wu:fb36g03 ELISA Kit, pcna-A ELISA Kit, proliferating cell nuclear antigen ELISA Kit, DNA polymerase III sliding clamp ELISA Kit, Proliferating cell nuclear antigen ELISA Kit, Proliferating cell nuclear antigen-like ELISA Kit, proliferating cell nuclear antigen L homeolog ELISA Kit, proliferating cell nuclear antigen 1 ELISA Kit, PCNA ELISA Kit, Pcna ELISA Kit, pcna ELISA Kit, MMP_RS08810 ELISA Kit, ANI_1_512014 ELISA Kit, AOR_1_1500154 ELISA Kit, MGC53867 ELISA Kit, pcna.L ELISA Kit, pcna1 ELISA Kit, LOC106420145 ELISA Kit, pcn1 ELISA Kit
- 基因ID
- 5111
- UniProt
- P12004
- 途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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