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PCNA ELISA 试剂盒

PCNA 适用: 人 Colorimetric Sandwich ELISA 2.5-600 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4884109
发货至: 中国
  • 抗原 See all PCNA ELISA试剂盒
    PCNA (Proliferating Cell Nuclear Antigen (PCNA))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    2.5-600 ng/mL
    最低检测浓度
    2.5 ng/mL
    应用范围
    ELISA
    原理
    Human PCNA ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes Human PCNA
    灵敏度
    2.46 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all PCNA ELISA试剂盒
    PCNA (Proliferating Cell Nuclear Antigen (PCNA))
    别名
    PCNA (PCNA 产品)
    别名
    PCNAR ELISA Kit, Pcna/cyclin ELISA Kit, pcna ELISA Kit, AO090003000846 ELISA Kit, 53/13 ELISA Kit, CG9193 ELISA Kit, DmPCNA ELISA Kit, Dmel\\CG9193 ELISA Kit, MUS209 ELISA Kit, Mus209 ELISA Kit, PCNA ELISA Kit, PCNA/mus209 ELISA Kit, Pcna ELISA Kit, dPCNA ELISA Kit, l(2)02448 ELISA Kit, l(2)56F ELISA Kit, l(2)56Fa ELISA Kit, l(2)s1534 ELISA Kit, cb16 ELISA Kit, fb36g03 ELISA Kit, wu:fa28e03 ELISA Kit, wu:fb36g03 ELISA Kit, pcna-A ELISA Kit, proliferating cell nuclear antigen ELISA Kit, DNA polymerase III sliding clamp ELISA Kit, Proliferating cell nuclear antigen ELISA Kit, Proliferating cell nuclear antigen-like ELISA Kit, proliferating cell nuclear antigen L homeolog ELISA Kit, proliferating cell nuclear antigen 1 ELISA Kit, PCNA ELISA Kit, Pcna ELISA Kit, pcna ELISA Kit, MMP_RS08810 ELISA Kit, ANI_1_512014 ELISA Kit, AOR_1_1500154 ELISA Kit, MGC53867 ELISA Kit, pcna.L ELISA Kit, pcna1 ELISA Kit, LOC106420145 ELISA Kit, pcn1 ELISA Kit
    基因ID
    5111
    UniProt
    P12004
    途径
    Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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