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C-JUN ELISA 试剂盒

JUN 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4882360
发货至: 中国
  • 抗原 See all C-JUN (JUN) ELISA试剂盒
    C-JUN (JUN) (Jun Proto-Oncogene (JUN))
    适用
    • 22
    • 19
    • 17
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human c-Jun/AP1 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human LILRB4.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all C-JUN (JUN) ELISA试剂盒
    C-JUN (JUN) (Jun Proto-Oncogene (JUN))
    别名
    C-Jun (JUN 产品)
    别名
    AP-1 ELISA Kit, AP1 ELISA Kit, c-Jun ELISA Kit, fj36h07 ELISA Kit, wu:fj36h07 ELISA Kit, zgc:65863 ELISA Kit, Junc ELISA Kit, c-jun ELISA Kit, CG2275 ELISA Kit, D-Jun ELISA Kit, D-jun ELISA Kit, D-jun/Jra ELISA Kit, DJUN ELISA Kit, DJun ELISA Kit, Djun ELISA Kit, Dmel\\CG2275 ELISA Kit, JRA ELISA Kit, JUN ELISA Kit, Jun ELISA Kit, V ELISA Kit, cJun ELISA Kit, d-JRA ELISA Kit, d-Jun ELISA Kit, d-jun ELISA Kit, dAP-1 ELISA Kit, dJRA ELISA Kit, dJUN ELISA Kit, dJra ELISA Kit, dJun ELISA Kit, djun ELISA Kit, dm-Jun ELISA Kit, jra ELISA Kit, jun ELISA Kit, l(2)46Ef ELISA Kit, l(2)IA109 ELISA Kit, l(2R)IA109 ELISA Kit, Jun proto-oncogene, AP-1 transcription factor subunit ELISA Kit, jun proto-oncogene S homeolog ELISA Kit, jun proto-oncogene ELISA Kit, Jun-related antigen ELISA Kit, jun proto-oncogene L homeolog ELISA Kit, JUN ELISA Kit, Jun ELISA Kit, jun.S ELISA Kit, jun ELISA Kit, Jra ELISA Kit, jun.L ELISA Kit
    基因ID
    3725
    UniProt
    P05412
    途径
    MAPK Pathway, RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins
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