C-JUN ELISA 试剂盒
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- 抗原 See all C-JUN (JUN) ELISA试剂盒
- C-JUN (JUN) (Jun Proto-Oncogene (JUN))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
- 品牌
- TransAM®
- 样品类型
- Cell Extracts, Tissue Samples
- Analytical Method
- Quantitative
- 特异性
- TransAM AP-1 c-Jun Kits are tested for sensitivity in detecting c-Jun in nuclear extracts from K-562 or WI-38 VA13 cells that are either unstimulated or stimulated with TPA or TPA/Ionomycin.
- 产品特性
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Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. - 组件
- One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
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- Discover our best selling JUN ELISA Kit
- Top Product
- Discover our top product JUN ELISA Kit
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Jun Proto-Oncogene (JUN) ELISA Kit
JUN 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Jun Proto-Oncogene (JUN) ELISA KitJUN 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Jun Proto-Oncogene (JUN) ELISA KitJUN 适用: 小鼠 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Jun Proto-Oncogene (JUN) ELISA KitJUN 适用: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Jun Proto-Oncogene (JUN) ELISA KitJUN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Jun Proto-Oncogene (JUN) ELISA KitJUN 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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These extracts are diluted down to 0.625 μg/well and assayed using the TransAM AP-1 c-Jun Kit. The ratio of the signals from the stimulated over the unstimulated cells must be above 2. Lot No.18715036 was tested using K-562 cells. It was developed for 4 minutes and gave a ratio of 6.4 (Figure 1). The basal level of c-Jun expression, and this ratio, may vary depending on the cell type tested and the stimulation used. TransAM AP-1 c-Jun Kits are also tested for specificity in detecting c-Jun activation. TransAM AP-1 c-Jun assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated AP-1 consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents AP-1 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on AP-1 binding.
- 实验时间
- 5 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Except for the cell extract that must be kept at -80°C, the kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the phospho-c-Jun antibody,DTT, oligonucleotides, poly[d(I-C)] and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extract at -80°C. This product is guaranteed for 6 months from date of receipt.
- 有效期
- 6 months
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- 抗原 See all C-JUN (JUN) ELISA试剂盒
- C-JUN (JUN) (Jun Proto-Oncogene (JUN))
- 别名
- Ap-1 C-Jun (JUN 产品)
- 别名
- AP-1 ELISA Kit, AP1 ELISA Kit, c-Jun ELISA Kit, fj36h07 ELISA Kit, wu:fj36h07 ELISA Kit, zgc:65863 ELISA Kit, Junc ELISA Kit, c-jun ELISA Kit, CG2275 ELISA Kit, D-Jun ELISA Kit, D-jun ELISA Kit, D-jun/Jra ELISA Kit, DJUN ELISA Kit, DJun ELISA Kit, Djun ELISA Kit, Dmel\\CG2275 ELISA Kit, JRA ELISA Kit, JUN ELISA Kit, Jun ELISA Kit, V ELISA Kit, cJun ELISA Kit, d-JRA ELISA Kit, d-Jun ELISA Kit, d-jun ELISA Kit, dAP-1 ELISA Kit, dJRA ELISA Kit, dJUN ELISA Kit, dJra ELISA Kit, dJun ELISA Kit, djun ELISA Kit, dm-Jun ELISA Kit, jra ELISA Kit, jun ELISA Kit, l(2)46Ef ELISA Kit, l(2)IA109 ELISA Kit, l(2R)IA109 ELISA Kit, Jun proto-oncogene, AP-1 transcription factor subunit ELISA Kit, jun proto-oncogene S homeolog ELISA Kit, jun proto-oncogene ELISA Kit, Jun-related antigen ELISA Kit, jun proto-oncogene L homeolog ELISA Kit, JUN ELISA Kit, Jun ELISA Kit, jun.S ELISA Kit, jun ELISA Kit, Jra ELISA Kit, jun.L ELISA Kit
- 途径
- MAPK Pathway, RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins
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