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COL1A1 ELISA 试剂盒

COL1A1 适用: 人 Colorimetric Sandwich ELISA 0.469-30 ng/mL Plasma, Serum
产品编号 ABIN454769
发货至: 中国
  • 抗原 See all COL1A1 ELISA试剂盒
    COL1A1 (Collagen, Type I, alpha 1 (COL1A1))
    适用
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.469-30 ng/mL
    最低检测浓度
    0.469 ng/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the in vitro quantitative determination of human Col ? concentrations in serum, plasma and other biological fluids.
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This assay recognizes recombinant and natural human Col ?.
    交叉反应 (详细)
    No significant cross-reactivity or interference was observed.
    灵敏度
    < 0.117 ng/mL
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    产品特性
    Homo sapiens,Human,Collagen alpha-1(I) chain,Alpha-1 type I collagen,COL1A1
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    Discover our best selling COL1A1 ELISA Kit
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    Discover our top product COL1A1 ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to Col Ⅰ. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Col Ⅰ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Col Ⅰ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Col Ⅰ in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    限制
    仅限研究用
  • 储存条件
    4 °C/-20 °C
    储存方法
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • 抗原 See all COL1A1 ELISA试剂盒
    COL1A1 (Collagen, Type I, alpha 1 (COL1A1))
    别名
    COL1A1 (COL1A1 产品)
    别名
    OI4 ELISA Kit, Col1a-1 ELISA Kit, Cola-1 ELISA Kit, Cola1 ELISA Kit, Mov-13 ELISA Kit, Mov13 ELISA Kit, COLIA1 ELISA Kit, col1a1 ELISA Kit, MGC52532 ELISA Kit, COL1A1 ELISA Kit, COL1A2 ELISA Kit, alpha1(I) ELISA Kit, cb21 ELISA Kit, sb:cb21 ELISA Kit, sb:cb384 ELISA Kit, wu:fa99c12 ELISA Kit, wu:fb02c06 ELISA Kit, alpha3(I) ELISA Kit, col1a3 ELISA Kit, hm:zeh0348 ELISA Kit, wu:fa95h05 ELISA Kit, wu:fd02a10 ELISA Kit, wu:fj59a10 ELISA Kit, collagen type I alpha 1 chain ELISA Kit, collagen, type I, alpha 1 ELISA Kit, collagen, type I, alpha 1 S homeolog ELISA Kit, collagen alpha-1(I) chain ELISA Kit, collagen type I alpha 2 chain ELISA Kit, collagen, type I, alpha 1a ELISA Kit, collagen 1a1 ELISA Kit, collagen, type I, alpha 1b ELISA Kit, COL1A1 ELISA Kit, Col1a1 ELISA Kit, col1a1.S ELISA Kit, LOC397571 ELISA Kit, COL1A2 ELISA Kit, col1a1a ELISA Kit, col1a1 ELISA Kit, col1a1b ELISA Kit
    背景
    Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Type 1 collagen is the most abundant protein in the connective tissues, especially in tendon, skin and bone.
    途径
    Sensory Perception of Sound, Autophagy, Growth Factor Binding
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