COL1A1 ELISA 试剂盒
-
- 抗原 See all COL1A1 ELISA试剂盒
- COL1A1 (Collagen, Type I, alpha 1 (COL1A1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Collagen alpha-1 chain ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human LRRN1.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling COL1A1 ELISA Kit
- Top Product
- Discover our top product COL1A1 ELISA Kit
-
-
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all COL1A1 ELISA试剂盒
- COL1A1 (Collagen, Type I, alpha 1 (COL1A1))
- 别名
- Collagen alpha-1 Chain (COL1A1 产品)
- 别名
- OI4 ELISA Kit, Col1a-1 ELISA Kit, Cola-1 ELISA Kit, Cola1 ELISA Kit, Mov-13 ELISA Kit, Mov13 ELISA Kit, COLIA1 ELISA Kit, col1a1 ELISA Kit, MGC52532 ELISA Kit, COL1A1 ELISA Kit, COL1A2 ELISA Kit, alpha1(I) ELISA Kit, cb21 ELISA Kit, sb:cb21 ELISA Kit, sb:cb384 ELISA Kit, wu:fa99c12 ELISA Kit, wu:fb02c06 ELISA Kit, alpha3(I) ELISA Kit, col1a3 ELISA Kit, hm:zeh0348 ELISA Kit, wu:fa95h05 ELISA Kit, wu:fd02a10 ELISA Kit, wu:fj59a10 ELISA Kit, collagen type I alpha 1 chain ELISA Kit, collagen, type I, alpha 1 ELISA Kit, collagen, type I, alpha 1 S homeolog ELISA Kit, collagen alpha-1(I) chain ELISA Kit, collagen type I alpha 2 chain ELISA Kit, collagen, type I, alpha 1a ELISA Kit, collagen 1a1 ELISA Kit, collagen, type I, alpha 1b ELISA Kit, COL1A1 ELISA Kit, Col1a1 ELISA Kit, col1a1.S ELISA Kit, LOC397571 ELISA Kit, COL1A2 ELISA Kit, col1a1a ELISA Kit, col1a1 ELISA Kit, col1a1b ELISA Kit
- 基因ID
- 1277
- UniProt
- P02452
- 途径
- Sensory Perception of Sound, Autophagy, Growth Factor Binding
-