TNFRSF1A ELISA 试剂盒
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- 抗原 See all TNFRSF1A ELISA试剂盒
- TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
- 抗原表位
- AA 30-212
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFR1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- NSO, L30-A212
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: L30-A212 - Featured
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Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kit
TNFRSF1A 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Urine
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA KitTNFRSF1A 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA KitTNFRSF1A 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA KitTNFRSF1A 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA KitTNFRSF1A 适用: 猴 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA KitTNFRSF1A 适用: 小鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA KitTNFRSF1A 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA KitTNFRSF1A 适用: 大鼠 Colorimetric Sandwich ELISA 2-600 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA KitTNFRSF1A 适用: 人 Soluble Colorimetric Sandwich ELISA 7.81-500 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse TNFR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFR1 has been precoated onto 96-well plates. Standards (NSO, L30-A212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFR1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TNFR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFR1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 46, Standard deviation: 2.7, CV(%): 5.9
- Sample 2: n=16, Mean(pg/ml): 118, Standard deviation: 6.5, CV(%): 5.5
- Sample 3: n=16, Mean(pg/ml): 581, Standard deviation: 27.5, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 48.3, Standard deviation: 2.5, CV(%): 5.2
- Sample 2: n=24, Mean(pg/ml): 120, Standard deviation: 6, CV(%): 5
- Sample 3: n=24, Mean(pg/ml): 592, Standard deviation: 33.3, CV(%): 5.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all TNFRSF1A ELISA试剂盒
- TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
- 别名
- TNFRSF1A (TNFRSF1A 产品)
- 别名
- CD120a ELISA Kit, FPF ELISA Kit, MS5 ELISA Kit, TBP1 ELISA Kit, TNF-R ELISA Kit, TNF-R-I ELISA Kit, TNF-R55 ELISA Kit, TNFAR ELISA Kit, TNFR1 ELISA Kit, TNFR1-d2 ELISA Kit, TNFR55 ELISA Kit, TNFR60 ELISA Kit, p55 ELISA Kit, p55-R ELISA Kit, p60 ELISA Kit, TNF-R1 ELISA Kit, TNF-alphaR1 ELISA Kit, TNFRI ELISA Kit, TNFRp55 ELISA Kit, TNFalpha-R1 ELISA Kit, Tnfr-2 ELISA Kit, Tnfr1 ELISA Kit, fpf ELISA Kit, tbp1 ELISA Kit, p55-r ELISA Kit, tnf-r ELISA Kit, tnfar ELISA Kit, tnfr1 ELISA Kit, cd120a ELISA Kit, tnfr55 ELISA Kit, tnfr60 ELISA Kit, xtnfr1 ELISA Kit, wu:fj65b10 ELISA Kit, zgc:85914 ELISA Kit, TNFR I ELISA Kit, TNFRSF1A ELISA Kit, TNFA ELISA Kit, cTNF ELISA Kit, TNF receptor superfamily member 1A ELISA Kit, tumor necrosis factor receptor superfamily, member 1a ELISA Kit, TNF receptor superfamily member 1A S homeolog ELISA Kit, tumor necrosis factor ELISA Kit, TNFRSF1A ELISA Kit, Tnfrsf1a ELISA Kit, tnfrsf1a.S ELISA Kit, tnfrsf1a ELISA Kit, TNF ELISA Kit
- 背景
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Protein Function: Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis (By similarity). .
Background: Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNF alpha). The protein encoded by this gene is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. And this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate the transcription factor NF-i°B, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.
Synonyms: Tumor necrosis factor receptor superfamily member 1A,Tumor necrosis factor receptor 1,TNF-R1,Tumor necrosis factor receptor type I,TNF-RI,TNFR-I,p55,p60,CD120a,Tnfrsf1a,Tnfr-1, Tnfr1,
Full Gene Name: Tumor necrosis factor receptor superfamily member 1A
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. Golgi apparatus membrane, Single-pass type I membrane protein. - 基因ID
- 21937
- UniProt
- P25118
- 途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway
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