TRAIL ELISA 试剂盒
(1 reference)-
- 抗原 See all TRAIL (TNFSF10) ELISA试剂盒
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 2-600 pg/mL
- 最低检测浓度
- 2 pg/mL
- 应用范围
- ELISA
- 原理
- Mouse TRAIL (TNFSF10) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects mouse TRAIL. Other species not determined.
- 灵敏度
- 2 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA Kit
TNFSF10 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 猴 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Serum, Urine
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitWide Range TNFSF10 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 人 AA 95-281 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA KitTNFSF10 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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: "The Critical Role of Proteolytic Relay through Cathepsins B and E in the Phenotypic Change of Microglia/Macrophage." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 36, pp. 12488-501, (2015) (PubMed).
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: "The Critical Role of Proteolytic Relay through Cathepsins B and E in the Phenotypic Change of Microglia/Macrophage." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 36, pp. 12488-501, (2015) (PubMed).
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- 抗原 See all TRAIL (TNFSF10) ELISA试剂盒
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- 别名
- TRAIL (TNFSF10 产品)
- 别名
- APO2L ELISA Kit, Apo-2L ELISA Kit, CD253 ELISA Kit, TL2 ELISA Kit, TRAIL ELISA Kit, A330042I21Rik ELISA Kit, AI448571 ELISA Kit, APO-2L ELISA Kit, Ly81 ELISA Kit, Trail ELISA Kit, tl2 ELISA Kit, apo2l ELISA Kit, cd253 ELISA Kit, trail ELISA Kit, apo-2l ELISA Kit, trail1 ELISA Kit, xtrail1 ELISA Kit, tnfsf10l2 ELISA Kit, zgc:92320 ELISA Kit, TNLG6A ELISA Kit, TNF superfamily member 10 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 10 ELISA Kit, tumor necrosis factor superfamily member 10 ELISA Kit, TNF superfamily member 10 S homeolog ELISA Kit, TNFSF10 ELISA Kit, Tnfsf10 ELISA Kit, tnfsf10.S ELISA Kit, tnfsf10 ELISA Kit
- 背景
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Gene Names: Tnfsf10 Trail
Protein names: Tumor necrosis factor ligand superfamily member 10 (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253)
- 基因ID
- 22035
- UniProt
- P50592
- 途径
- Apoptosis, Positive Regulation of Endopeptidase Activity
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