PD-1 ELISA 试剂盒
(1 reference)-
- 抗原 See all PD-1 (PDCD1) ELISA试剂盒
- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 20-6.000 pg/mL
- 最低检测浓度
- 20 pg/mL
- 应用范围
- ELISA
- 原理
- Human PD-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human PD-1. Other species not determined yet.
- 灵敏度
- 20 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling PDCD1 ELISA Kit
- Top Product
- Discover our top product PDCD1 ELISA Kit
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Programmed Cell Death 1 (PDCD1) ELISA Kit
PDCD1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: Various Species Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 AA 25-167 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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: "Serum levels of soluble programmed death-1 and programmed death ligand-1 in systemic sclerosis: Association with extent of skin sclerosis." in: The Journal of dermatology, Vol. 43, Issue 8, pp. 954-7, (2016) (PubMed).
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: "Serum levels of soluble programmed death-1 and programmed death ligand-1 in systemic sclerosis: Association with extent of skin sclerosis." in: The Journal of dermatology, Vol. 43, Issue 8, pp. 954-7, (2016) (PubMed).
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- 抗原 See all PD-1 (PDCD1) ELISA试剂盒
- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
- 别名
- PD-1 (PDCD1 产品)
- 别名
- CD279 ELISA Kit, PD-1 ELISA Kit, PD1 ELISA Kit, SLEB2 ELISA Kit, hPD-1 ELISA Kit, hPD-l ELISA Kit, Ly101 ELISA Kit, Pdc1 ELISA Kit, programmed cell death 1 ELISA Kit, PDCD1 ELISA Kit, Pdcd1 ELISA Kit
- 背景
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Gene Names: PDCD1 PD1
Protein names: Programmed cell death protein 1 (Protein PD-1) (hPD-1) (CD antigen CD279)
- 基因ID
- 5133
- UniProt
- Q15116
- 途径
- Cancer Immune Checkpoints
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