PD-1 ELISA 试剂盒
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- 抗原 See all PD-1 (PDCD1) ELISA试剂盒
- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
- 抗原表位
- AA 25-167
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PD-1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: L25-Q167 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: L25-Q167 - Featured
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Programmed Cell Death 1 (PDCD1) ELISA Kit
PDCD1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: Various Species Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human PD-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PD-1 has been precoated onto 96-well plates. Standards(NSO, L25-Q167) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PD-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PD-1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PD-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PD-1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1482, Standard deviation: 77.1, CV(%): 5.2
- Sample 2: n=16, Mean(pg/ml): 2838, Standard deviation: 178.8, CV(%): 6.3
- Sample 3: n=16, Mean(pg/ml): 5430, Standard deviation: 347.52, CV(%): 6.4,
- Sample 1: n=24, Mean(pg/ml): 1326, Standard deviation: 87.52, CV(%): 6.6
- Sample 2: n=24, Mean(pg/ml): 3263, Standard deviation: 238.2, CV(%): 7.3
- Sample 3: n=24, Mean(pg/ml): 6102, Standard deviation: 470, CV(%): 7.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all PD-1 (PDCD1) ELISA试剂盒
- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
- 别名
- PDCD1 (PDCD1 产品)
- 别名
- CD279 ELISA Kit, PD-1 ELISA Kit, PD1 ELISA Kit, SLEB2 ELISA Kit, hPD-1 ELISA Kit, hPD-l ELISA Kit, Ly101 ELISA Kit, Pdc1 ELISA Kit, programmed cell death 1 ELISA Kit, PDCD1 ELISA Kit, Pdcd1 ELISA Kit
- 背景
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Protein Function: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen- specific manner. Possible cell death inducer, in association with other factors. .
Background: Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
Synonyms: Programmed cell death protein 1,Protein PD-1,hPD-1,CD279,PDCD1,PD1,
Full Gene Name: Programmed cell death protein 1
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 5133
- UniProt
- Q15116
- 途径
- Cancer Immune Checkpoints
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