PD-1 ELISA 试剂盒
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Quick Overview for PD-1 ELISA 试剂盒 (ABIN6719831)
抗原
See all PD-1 (PDCD1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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Supplier Product No.
- koa0589
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Supplier
- Rockland
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原理
- Human PD-1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: L25-Q167 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Programmed Cell Death 1 (PDCD1) ELISA Kit
PDCD1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: Various Species Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 AA 25-167 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma (heparin), Serum
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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说明
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Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PD-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates to each empty well. It is recommended that each human PD-1 standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.2%, 5.1%, 5.2%
Inter-Assay Precision (CV%): 5.1%, 5.2%, 6.3% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
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别名
- PD-1
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背景
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Background: Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
Gene Full Name: programmed cell death 1
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基因ID
- 5133
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NCBI登录号
- NP_005009
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UniProt
- Q15116
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途径
- Cancer Immune Checkpoints
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