IL-1 beta ELISA 试剂盒
Quick Overview for IL-1 beta ELISA 试剂盒 (ABIN2648759)
抗原
See all IL-1 beta (IL1B) ELISA试剂盒适用
检测方法
应用范围
样品类型
-
-
灵敏度
- 2 pg/ml
-
产品特性
- Interleukin-1 (IL-1), originally described as lymphocyte activating factor (LAF) for its effects on thymocytes (1), is a polypeptide cytokine with two molecular forms. The two distinct molecular forms of IL-1 are thought to be derived from two genes (2,3). After transcription, as 31kD precursor polypeptide is cleaved to give rise to mostly cell membrane associated IL-1a and secreted IL-1b. Both have the same molecular weight of 15kD but have different isoelectric points of 5 and 7, respectively.
-
-
Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 AA 117-269 Colorimetric Sandwich ELISA 3.9-250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鼠 AA 118-269 Colorimetric Sandwich ELISA 12.5-800 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 AA 117-268 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine
Interleukin 1, beta (IL1B) ELISA KitHigh Sensitivity IL1B 适用: 兔 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: Pig Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
样本量
- 100 μL
-
实验时间
- 3.5 h
-
板类型
- Pre-coated
-
实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1b present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1βis added to the wells and binds to the combination of capture antibody-IL-1βin sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-1β present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1β standard dilutions and IL-1β sample concentration determined
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
-
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
-
别名
- IL-1beta
-
途径
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
抗原 See all IL-1 beta (IL1B) ELISA试剂盒
-
