C-JUN ELISA 试剂盒
Quick Overview for C-JUN ELISA 试剂盒 (ABIN2044773)
抗原
See all C-JUN (JUN) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.051 ng/mL
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板类型
- Pre-coated
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实验流程
- V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- C-JUN (JUN) (Jun Proto-Oncogene (JUN))
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别名
- V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)
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途径
- MAPK Pathway, RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins
抗原 See all C-JUN (JUN) ELISA试剂盒
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