IL1RN ELISA 试剂盒
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- 抗原 See all IL1RN ELISA试剂盒
- IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))
- 抗原表位
- AA 27-178
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-1RA/IL1RN
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: R27-Q178 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: R27-Q178 - Featured
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- Top Product
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Interleukin 1 Receptor Antagonist (IL1RN) ELISA Kit
IL1RN 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 犬 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 马 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor Antagonist (IL1RN) ELISA KitIL1RN 适用: 猴 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-1RA has been precoated onto 96-well plates. Standards(E.coli, R27-Q178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1RA amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-1RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-1RA standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 476, Standard deviation: 30, CV(%): 6.3
- Sample 2: n=16, Mean(pg/ml): 1326, Standard deviation: 72.93, CV(%): 5.5
- Sample 3: n=16, Mean(pg/ml): 2760, Standard deviation: 129.72, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 595, Standard deviation: 46.41, CV(%): 7.8
- Sample 2: n=24, Mean(pg/ml): 1533, Standard deviation: 95, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 3185, Standard deviation: 172, CV(%): 5.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all IL1RN ELISA试剂盒
- IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))
- 别名
- IL1RN (IL1RN 产品)
- 别名
- DIRA ELISA Kit, ICIL-1RA ELISA Kit, IL-1RN ELISA Kit, IL-1ra ELISA Kit, IL-1ra3 ELISA Kit, IL1F3 ELISA Kit, IL1RA ELISA Kit, IRAP ELISA Kit, MVCD4 ELISA Kit, IRAP1 ELISA Kit, IL1RN ELISA Kit, IL-1RA ELISA Kit, F630041P17Rik ELISA Kit, interleukin 1 receptor antagonist ELISA Kit, interleukin 1 receptor antagonist-like ELISA Kit, IL1RN ELISA Kit, Il1rn ELISA Kit, IL1RNL ELISA Kit
- 背景
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Protein Function: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
Background: The interleukin-1 receptor antagonist(IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization. IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha(IL1A) and interleukin 1, beta(IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.
Synonyms: Interleukin-1 receptor antagonist protein,IL-1RN,IL-1ra,IRAP,IL1 inhibitor,Il1rn,Il-1ra,
Full Gene Name: Interleukin-1 receptor antagonist protein
Cellular Localisation: Isoform 1: Secreted. - 基因ID
- 16181
- UniProt
- P25085
- 途径
- NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints
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