IL1R1 ELISA 试剂盒
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- 抗原 See all IL1R1 ELISA试剂盒
- IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))
- 抗原表位
- AA 21-332
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 125-8000 pg/mL
- 最低检测浓度
- 125 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL1R1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: D21-T332 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: D21-T332 - Featured
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- Discover our top product IL1R1 ELISA Kit
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Interleukin 1 Receptor, Type I (IL1R1) ELISA Kit
IL1R1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor, Type I (IL1R1) ELISA KitIL1R1 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Receptor, Type I (IL1R1) ELISA KitIL1R1 适用: 人 Colorimetric Sandwich ELISA 6-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the interleukin-1 receptor family.
- 板类型
- Pre-coated
- 实验流程
- human IL1R1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1R1 has been precoated onto 96-well plates. Standards(NSO, D21-T332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1R1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1R1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human IL1R1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL1R1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 746, Standard deviation: 30.59, CV(%): 4.1
- Sample 2: n=16, Mean(pg/ml): 3374, Standard deviation: 175.5, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 5587, Standard deviation: 312.9, CV(%): 5.6,
- Sample 1: n=24, Mean(pg/ml): 857, Standard deviation: 59.13, CV(%): 6.9
- Sample 2: n=24, Mean(pg/ml): 3345, Standard deviation: 250.9, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 6558, Standard deviation: 518.1, CV(%): 7.9
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all IL1R1 ELISA试剂盒
- IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))
- 别名
- IL1R1 (IL1R1 产品)
- 别名
- CD121A ELISA Kit, D2S1473 ELISA Kit, IL-1R-alpha ELISA Kit, IL1R ELISA Kit, IL1RA ELISA Kit, P80 ELISA Kit, CD121a ELISA Kit, CD121b ELISA Kit, IL-iR ELISA Kit, Il1r-1 ELISA Kit, DER4 ELISA Kit, Fit-1 ELISA Kit, Ly84 ELISA Kit, ST2L ELISA Kit, St2 ELISA Kit, St2-rs1 ELISA Kit, T1 ELISA Kit, T1/ST2 ELISA Kit, interleukin 1 receptor type 1 ELISA Kit, interleukin 1 receptor, type I ELISA Kit, interleukin 1 receptor-like 1 ELISA Kit, IL1R1 ELISA Kit, Il1r1 ELISA Kit, Il1rl1 ELISA Kit
- 背景
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Protein Function: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. .
Background: Interleukin 1 receptor, type I(IL1R1), also known as CD121a(Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. IL1R1 along with interleukin 1 receptor, type II(IL1R2), interleukin 1 receptor-like 2(IL1RL2), and interleukin 1 receptor-like 1(IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. This protein is a receptor for interleukin 1 alpha(IL1A), interleukin 1 beta(IL1B), and interleukin 1 receptor antagonist(IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses.
Synonyms: Interleukin-1 receptor type 1,IL-1R-1,IL-1RT-1,IL-1RT1,CD121 antigen-like family member A,Interleukin-1 receptor alpha,IL-1R-alpha,Interleukin-1 receptor type I,p80,CD121a,Interleukin-1 receptor type 1, membrane form,mIL-1R1,mIL-1RI,Interleukin-1 receptor type 1, soluble form,sIL-1R1,sIL-1RI,IL1R1,IL1R, IL1RA, IL1RT1,
Full Gene Name: Interleukin-1 receptor type 1
Cellular Localisation: Membrane, Single-pass type I membrane protein . Cell membrane . Secreted. - 基因ID
- 3554
- UniProt
- P14778
- 途径
- NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints
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