IL-1 beta ELISA 试剂盒
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Quick Overview for IL-1 beta ELISA 试剂盒 (ABIN1112642)
抗原
See all IL-1 beta (IL1B) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- For quantitative detection of IL-1β in rat serum , plasma, urine, cell culture supernatant,tissue samples.
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Analytical Method
- Quantitative
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组件
- 1. One 96-well plate pre-coated with anti-Mouse IL-1beta antibody 2. Lyophilized Mouse IL-1beta standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse IL-1beta antibody (Concentrated): 130 µl.
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试剂未包括
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- 37 °C incubator
- Microplate reader (wavelength: 450 nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5 mL of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1 L
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Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 AA 117-269 Colorimetric Sandwich ELISA 3.9-250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 AA 117-268 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鼠 AA 118-269 Colorimetric Sandwich ELISA 12.5-800 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 鱼 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鸡 Colorimetric Competition ELISA 0.27 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-1? polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-1? polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the IL-1? amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of IL-1? can be calculated.
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板类型
- Pre-coated
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试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37 °C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
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样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernatants or body fluids: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C° C.
Serum: Coagulate the serum at room temperature (about 4 hours) or coat at 4°C overnight. Centrifuge at approximately 1500 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with EDTA as the anticoagulant. Centrifuge for 15 min at 1500 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate or heparin can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. -
限制
- 仅限研究用
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储存液
- Sodium azide, Thimerosal (Merthiolate)
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C for 6 months.
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有效期
- 12 months
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- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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别名
- IL-1beta
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背景
- Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. It can reset glucose homeostasis at central levels. It cause oligodendrocyte death in coculture with astrocytes and microglia, and also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Pathak et al. (2011) proposed that IL1B blockade may be a viable therapy for patients with autoimmune inner ear disease or sensorineural hearing loss that fail to respond to corticosteroids.
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途径
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
抗原 See all IL-1 beta (IL1B) ELISA试剂盒
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