anti-人 CD11b 抗体 for Immunoprecipitation

Recommended CD11b Antibody (supplied by: Log in to see )

抗原
Integrin alpha M (ITGAM) 抗体
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
适用

675
468
172
79
31
17
15
14
13
11
10
9
7
7
5
4
4
4
2
2
宿主
小鼠
503
388
143
16
5
2
克隆类型
单克隆
标记
This CD11b antibody is un-conjugated
147
123
89
62
19
18
15
15
14
14
13
12
12
11
9
8
7
7
7
6
6
6
5
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
应用范围
Flow Cytometry (FACS), Immunoprecipitation (IP)
867
228
227
172
160
138
100
96
90
68
21
21
14
13
12
8
5
5
4
3
3
1
1
1
1
1
1
1
1
1
选项
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产品编号 ABIN125683
$ 253.00
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.90531 ABIN1302538 FACS IP APC Mouse IgG2a Log in to see MEM-174 4
23.90531 ABIN93959 FACS IP FITC Mouse IgG2a Log in to see MEM-174 5
23.90531 ABIN93958 FACS IP Biotin Mouse IgG2a Log in to see MEM-174 5
23.90531 ABIN93960 FACS IP PE Mouse IgG2a Log in to see MEM-174 5
23.90531 ABIN93956 FACS IP Mouse IgG2a Log in to see MEM-174 5
1 ABIN2688906 BR IHC (fro) FACS IHC (p) IP WB Rat IgG2b, kappa Log in to see M1-70 8
1 ABIN118146 FACS IHC (fro) IF IHC (p) IP WB Rat IgG2b Log in to see M1-70-15 18
1 ABIN120130 FACS IHC (fro) IF IP Rat IgG2b Log in to see 5C6 13
1 ABIN2749103 Func IHC (f) ICC FACS IP PE Mouse IgG1 Log in to see ICRF44 4
1 ABIN118145 FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70 14
1 ABIN118516 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 5
1 ABIN320196 FACS IHC (fro) IP Rat IgG2b Log in to see 5C6 13
1 ABIN1690733 FACS Func IHC (f) IP APC Rat IgG2b Log in to see M1-70 10
1 ABIN2749102 Func IHC (f) ICC FACS IP FITC Mouse IgG1 Log in to see ICRF44 4
1 ABIN2659829 FACS IP PerCP Rat IgG2b kappa Log in to see M1-70 11
1 ABIN1450195 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 12
1 ABIN118517 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
1 ABIN2749012 Func IHC (f) ICC FACS IP Mouse IgG1 Log in to see ICRF44 3
1 ABIN2659830 FACS IP PerCP Rat IgG2b kappa Log in to see M1-70 12
1 ABIN118513 FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4

General

抗原 Integrin alpha M (ITGAM) 抗体
适用
(675), (468), (172), (79), (31), (17), (15), (14), (13), (11), (10), (9), (7), (7), (5), (4), (4), (4), (2), (2)
宿主 小鼠
(503), (388), (143), (16), (5), (2)
克隆类型 (克隆位点)
单克隆   ( )
标记 This CD11b antibody is un-conjugated
(147), (123), (89), (62), (19), (18), (15), (15), (14), (14), (13), (12), (12), (11), (9), (8), (7), (7), (7), (6), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Flow Cytometry (FACS), Immunoprecipitation (IP)
(867), (228), (227), (172), (160), (138), (100), (96), (90), (68), (21), (21), (14), (13), (12), (8), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5个引用可参考
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CD11b抗体详情

CD11b目标详情 使用细节 贮存及处理 References for anti-CD11b antibody (ABIN125683) 图像
特异性 The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
HLDA VI, WS Code BP 310
HLDA VI, WS Code M 18
纯化方法 Purified from hybridoma culture supernatant by protein A-affinity chromatography.
纯度 > 95 % (by SDS-PAGE)
过滤 0.2 μm filtered
免疫原 Human granulocytes
克隆位点 MEM-174
亚型 IgG2a

CD11b目标详情

CD11b抗体详情 使用细节 贮存及处理 References for anti-CD11b antibody (ABIN125683) 图像 回页首
抗原
别名 ITGAM (ITGAM Antibody 摘要)
背景 CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
途径 Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

使用细节

CD11b抗体详情 CD11b目标详情 贮存及处理 References for anti-CD11b antibody (ABIN125683) 图像 回页首
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用

贮存及处理

CD11b抗体详情 CD11b目标详情 使用细节 References for anti-CD11b antibody (ABIN125683) 图像 回页首
浓度 1 mg/mL
缓冲液 Azide free phosphate buffered saline (PBS), approx. pH 7.4.
储存液 Azide free
注意事项 Do not freeze.
储存条件 4 °C
储存方法 Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD11b antibody (ABIN125683)

CD11b抗体详情 CD11b目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, 2015

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007

背景

Lawrence, Srikumaran: "CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization." in: Veterinary immunology and immunopathology, Vol. 119, Issue 3-4, pp. 287-98, 2007

Akramiene, Kondrotas, Didziapetriene, Kevelaitis: "Effects of beta-glucans on the immune system." in: Medicina (Kaunas, Lithuania), Vol. 43, Issue 8, pp. 597-606, 2007

Hentzen, Neelamegham, Kansas, Benanti, McIntire, Smith, Simon: "Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1." in: Blood, Vol. 95, Issue 3, pp. 911-20, 2000

图像

CD11b抗体详情 CD11b目标详情 使用细节 贮存及处理 References for anti-CD11b antibody (ABIN125683) 回页首
厂商提供的图像
Immunoprecipitation (IP) image for anti-Integrin alpha M (ITGAM) antibody (ABIN125683) Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC is...