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CD11b 抗体

Name: CD11b 抗体
Brand: antibodies-online
SKU: ABIN125683
Availability: OutOfStock

ITGAM 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-174 unconjugated

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD11b (MEM-174) purified antibody (concentration in sample 0,3 μg/mL, GAM APC).

FACS

Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor.

Separation of human monocytes (red-filled) from CD11b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11b (MEM-174) purified antibody (concentration in sample 0,3 μg/mL, GAM APC).

FACS

3 images

(2 references)

产品编号 ABIN125683

发货至: 中国

Datasheet as PDF

抗原 See all CD11b (ITGAM) 抗体

CD11b (ITGAM) (Integrin alpha M (ITGAM))
适用
257

168

62

33

17

17

16

14

11

7

7

5

5

2

2

1
人
宿主
136

104

103

4

1

1
小鼠
Compatible Secondaries
克隆类型
249

96

1
单克隆
标记
118

37

29

26

20

10

7

6

6

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5

5

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This CD11b antibody is un-conjugated
应用范围
259

83

75

71

59

40

36

27

22

20

13

13

7

6

5

5

4

3

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Flow Cytometry (FACS), Immunoprecipitation (IP)

特异性

The antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

交叉反应（详细）

Human

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Human granulocytes

克隆位点

MEM-174

亚型

IgG2a

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anti-Integrin alpha M (ITGAM) antibody (Biotin)
ITGAM 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-174 Biotin

anti-Integrin alpha M (ITGAM) antibody (FITC)
ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 FITC

anti-Integrin alpha M (ITGAM) antibody (PE)
ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 PE

应用备注

Flow cytometry: Recommended dilution: 1 μg/mL, positive control: peripheral blood lymphocytes.
Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants.
Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 mL of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4

储存液

Azide free

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, (2015) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).
抗原

CD11b (ITGAM) (Integrin alpha M (ITGAM))

别名

CD11b (ITGAM 产品)

别名

CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody

背景

Integrin subunit alpha M,CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.,Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

基因ID

3684

UniProt

P11215

途径

Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation