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Western blot抗体 | 经验证一抗

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527,879 results

AKT 1/2/3 抗体 (AA 401-479)

适用: 人, 小鼠, 大鼠, Cow, Pig WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Flow Cytometry validation image for anti-AKT 1/2/3 (AA 401-479) antibody (ABIN725195)

FACS

Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Mouse placenta lysate with Anti-AKT1/3 Polyclonal Antibody Unconjugated at 1:5000 for 90 min at 37˚C.

Lane 1: Mouse Placenta lysates；Lane 2: Mouse Lung lysates probed with AKT1/3 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

5 images

9 references

1 validation

产品编号 ABIN725195

100 μL

发货至: 中国

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详细

Independently Validated

PD-1 抗体 (AA 201-288)

PDCD1 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Programmed Cell Death 1 (PDCD1) (AA 201-288) antibody (ABIN735608)

5 images

2 references

1 validation

产品编号 ABIN735608

100 μL

发货至: 中国

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详细

Independently Validated

IDE 抗体 (AA 491-590)

IDE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Insulin-Degrading Enzyme (IDE) (AA 491-590) antibody (ABIN723680)

8 images

2 validations

产品编号 ABIN723680

100 μL

发货至: 中国

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详细

Independently Validated

TNFSF9 抗体 (AA 101-200)

TNFSF9 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

IHC

Independently Validated Antibody, image provided by Science Direct, badge number 029578:Formalin-fixed and paraffin embedded human liver labeled with Anti-TNFSF9/CD137L Polyclonal Antibody, Unconjugated (ABIN705056) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-TNFSF9/CD137L Polyclonal Antibody, Unconjugated (ABIN705056) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Images provided the Independent Validation Program, badge number 029578:Formalin-fixed and paraffin embedded human liver labeled with Anti-TNFSF9/CD137L Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

9 images

1 validation

产品编号 ABIN705056

100 μL

发货至: 中国

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详细

Independently Validated

PI3K p85 alpha/gamma 抗体 (pTyr199, pTyr467)

PI3K p85a/g 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g) (pTyr199), (pTyr467) antibody (ABIN744743)

Image provided by Independent Validation Program, badge 29801.

Formalin-fixed and paraffin embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

5 images

3 references

1 validation

产品编号 ABIN744743

100 μL

发货至: 中国

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详细

Independently Validated

ATM 抗体 (pSer1981)

Rockland 200-301-400 ATM 适用: 人 WB, IHC, ELISA, IF, IP, FACS, FM 宿主: 小鼠 Monoclonal 10H11-E12 unconjugated

Anti-ATMpS1981 Flow Cytometry Flow Cytometry of Mouse anti-ATMpS1981 antibody.

FACS

Anti-ATM Monoclonal Antibody - Immunofluorescence Microscopy anti-ATM pS1981 mouse monoclonal antibody (Catalog # 200-301-400) detects ATM phosphorylated on Ser 1981 by Indirect immunofluorescence microscopy.

Anti-ATM Monoclonal Antibody - Immunofluorescence Microscopy Anti ATM antibody showing overlay of anti-ATM pS1981 staining.

Anti-ATM Monoclonal Antibody-Immunohistochemistry.

ATM is activated by spermidine in GM00637 cells.

ICC

12 images

126 references

产品编号 ABIN6656104

100 μg

发货至: 中国

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详细

Rockland Original

BAX 抗体 (AA 84-175)

BAX 适用: 人, 小鼠, 大鼠, Pig, Cow, 兔, 小鸡, 斑马鱼 WB, ELISA, FACS, IP, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

L1 rat brain, L2 mouse liver lysates probed at 1:200 in 4˚C.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (ABIN725390) at 1:800 followed by conjugation to the secondary antibody and DAB staining

11 images

34 references

产品编号 ABIN725390

100 μL

发货至: 中国

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详细

NF-kB p65 抗体 (AA 51-100)

NFkBP65 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 小鸡 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rat aortic tissue labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (ABIN668961), Unconjugated at 1:600 followed by conjugation to the secondary antibody and DAB staining

7 images

30 references

产品编号 ABIN668961

100 μL

发货至: 中国

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详细

CD8 抗体 (AA 51-150)

CD8 适用: 人, 小鼠, 大鼠 FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD8 Polyclonal Antibody .

FACS

$Quantification and representative immunohistological staining of immune cells in the fracture haematoma/callus.$ IHC (p)

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN671391) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN671391) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C.

Image kindly submitted by Amy Beck as part of the free sample program.

IHC (p)

Mouse spleen lysate probed with Anti-CD8 Polyclonal Antibody at 1:5000 90min in 37˚C.

H&E and IHC staining for CD8, IL-12 and IFN-γ in subcutaneous LLC1 tumors.

IHC

7 images

26 references

产品编号 ABIN671391

100 μL

发货至: 中国

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详细

CD163 抗体 (AA 1001-1121)

CD163 适用: 人 WB, FACS, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti CD163 Polyclonal Antibody, Unconjugated (ABIN741570) at 1:200 followed by conjugation to the secondary antibody and DAB staining

7 images

27 references

产品编号 ABIN741570

100 μL

发货至: 中国

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详细

Reelin 抗体 (AA 3143-3461)

RELN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Reelin (RELN) (AA 3143-3461) antibody (ABIN7435136)

IHC

Detection of RELN in Mouse Fetal rat lysate using Polyclonal Antibody to Reelin (RELN)

Detection of Recombinant RL, Mouse using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Mouse Liver Tissue using Polyclonal Antibody to Reelin (RELN)

IHC

Detection of RELN in Mouse Cerebrum Tissue using Polyclonal Antibody to Reelin (RELN)

IHC

Detection of RELN in Mouse Serum using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Human HepG2 cell lysate using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Mouse Cerebrum lysate using Polyclonal Antibody to Reelin (RELN)

8 images

1 validation

产品编号 ABIN7435136

100 μL (More quantities available)

发货至: 中国

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详细

Independently Validated

Cortactin 抗体 (N-Term)

KO Validated CTTN 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

Multiplex Immunohistochemistry validation image for anti-Cortactin (CTTN) (N-Term) antibody (ABIN2854674)

mIHC

WB Image Sample (30 ug of whole cell lysate) A: PC-12 7.

WB Image Sample (30 ug of whole cell lysate) A: Hep G2 , 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cortactin, antibody at 1:250 dilution.

IHC

ICC/IF Image Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunofluorescent analysis.

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.

7 images

1 validation

产品编号 ABIN2854674

100 μL

发货至: 中国

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详细

Independently Validated

APP 抗体 (C-Term)

APP 适用: 人 WB, IF, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (C-Term) antibody (ABIN2855011)

IHC

WB Image Sample(30 μg of whole cell lysate) A:Hep G2, 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded human tonsil, plasma cells, using APP, antibody(10 μg/ml).

IHC

ICC/IF Image APP antibody [C2C3], C-term detects APP protein at cytoplasm and nucleus by immunofluorescent analysis.

IHC-P Image APP antibody [C2C3], C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis.

IHC

WB Image Various whole cell extracts (30 μg) were separated by 7.

WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 7.

ICC/IF Image APP antibody [C2C3], C-term detects APP protein at cytoplasm by confocal immunofluorescent analysis.

8 images

1 validation

产品编号 ABIN2855011

100 μL

发货至: 中国

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详细

Independently Validated

ARPC2 抗体

ARPC2 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

Multiplex Immunohistochemistry validation image for anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) antibody (ABIN2855249)

mIHC

WB Image ARPC2 antibody detects ARPC2 protein by Western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ARPC2, antibody at 1:100 dilution.

IHC

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2, antibody at 1:200 dilution.

WB Image Sample(30 μg of whole cell lysate) A:293T B: HeLa S3, 12% SDS PAGE antibody diluted at 1:500

7 images

1 validation

产品编号 ABIN2855249

100 μL

发货至: 中国

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详细

Independently Validated

GLI3 抗体

GLI3 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-GLI Family Zinc Finger 3 (GLI3) antibody (ABIN2855813)

CUT&RUN

WB Image GLI3 antibody detects GLI3 protein by Western blot analysis.

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 5% SDS PAGE antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3, antibody at 1:500 dilution.

IHC-P Image GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.

IHC

WB Image Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE antibody diluted at 1:1000

Expression changes of Hedgehog signaling components.

IHC

Expression of genes and proteins related to Hh signaling in SLC mice.

IHC

10 images

1 validation

产品编号 ABIN2855813

100 μL

发货至: 中国

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详细

Independently Validated

NFKB1 抗体 (AA 860-969)

NFKB1 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 4A11 unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 860-969) antibody (ABIN561981)

Western Blot detection against Immunogen (37.

Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell.

Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.

Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon.

ISt

Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.

ELISA

Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1.

PLA

8 images

1 validation

产品编号 ABIN561981

100 μg

发货至: 中国

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详细

Independently Validated

ETV4 抗体 (AA 1-207)

ETV4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Ets Variant 4 (ETV4) (AA 1-207) antibody (ABIN6131217)

CUT&RUN

Western blot analysis of extracts of various cell lines, using Pea3 / ETV4 Rabbit pAb (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at 1:1000 dilution.

Immunofluorescence analysis of U-2 OS cells using Pea3 / ETV4 Rabbit pAb (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

10 images

1 validation

产品编号 ABIN6140332

100 μL (More quantities available)

发货至: 中国

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详细

Independently Validated

Histone H4 抗体 (acLys12)

HISTH4 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Histone H4 (HISTH4) (acLys12) antibody (ABIN6254899)

IHC

Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Acetyl-Histone H4 (Lys12) Antibody.

IF/ICC

Western blot analysis of extracts from mouse spleen, using Acetyl-Histone H4 (Lys12) Antibody.

ABIN6267063 at 1/100 staining human Brain tissue sections by IHC-P.

IHC

Western blot analysis of extracts from various samples, Acetyl-Histone H4 (Lys12) Antibody.

6 images

1 validation

产品编号 ABIN6254899

100 μL (More quantities available)

发货至: 中国

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详细

Independently Validated

CREB1 抗体

CREB1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 5G3 unconjugated

Western Blotting validation image for anti-cAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061)

Western blot analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse mAb with DAB staining.

IHC

Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng),

ELISA

Immunofluorescence analysis of Hela cells using CREB1 mouse mAb (green).

5 images

3 references

1 validation

产品编号 ABIN969061

100 μL

发货至: 中国

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详细

Independently Validated

NeuN 抗体 (AA 1-140)

RBFOX3 适用: 人, 小鼠 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1H3B2 unconjugated

Western Blotting validation image for anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody (ABIN5542704)

Immunohistochemistry validation image for anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody (ABIN5542704)

IHC

Western blot analysis using RBFOX3 mAb against human RBFOX3 (AA: 1-140) recombinant protein.

Immunofluorescence analysis of Hela cells using RBFOX3 mouse mAb (green).

ICC

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

ELISA

Western blot analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using RBFOX3 mouse mAb against C2C12 (1), RAW264.

Flow cytometric analysis of Hela cells using RBFOX3 mouse mAb (green) and negative control (red).

FACS

8 images

2 validations

产品编号 ABIN5542704

100 μL

发货至: 中国

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详细

Independently Validated

SYN1 抗体 (AA 362-511)

SYN1 适用: 人, 大鼠, 小鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7H10G6 unconjugated

Multiplex Immunohistochemistry validation image for anti-Synapsin I (SYN1) (AA 362-511) antibody (ABIN5542390)

mIHC

Western blot analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein.

Immunohistochemical analysis of paraffin-embedded testis tissues using SYN1 mouse mAb with DAB staining.

IHC

Western blot analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using SYN1 mouse mAb against SK-N-SH (1), NIH/3T3 (2), U251 (3), C6 (4), A549 (5), MCF-7 (6), and COS7 (7) cell lysate.

Flow cytometric analysis of Hela cells using SYN1 mouse mAb (green) and negative control (red).

12 images

1 validation

产品编号 ABIN5542390

100 μL

发货至: 中国

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详细

Independently Validated

NK2 Homeobox 5 抗体

NKX2-5 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-NK2 Homeobox 5 (NKX2-5) antibody (ABIN2856166)

CUT&RUN

WB Image Sample(30 ug whole cell lysate) A:H1299 B:Raji , 10% SDS PAGE antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Nkx2.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NKX2-5, antibody at 1:200 dilution.

6 images

1 validation

产品编号 ABIN2856166

100 μL

发货至: 中国

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详细

Independently Validated

CBS 抗体 (AA 1-100)

CBS 适用: 人 WB, ELISA, IHC (p), IP, RNAi 宿主: 小鼠 Monoclonal 3E1 unconjugated

Western Blot detection against Immunogen (36.

Immunoprecipitation of CBS transfected lysate using anti-CBS monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with CBS MaxPab rabbit polyclonal antibody.

Immunoperoxidase of monoclonal antibody to CBS on formalin-fixed paraffin-embedded human hepatocellular carcinoma.

ISt

CBS monoclonal antibody (M01), clone 3E1 Western Blot analysis of CBS expression in HeLa .

Western Blot analysis of CBS expression in transfected 293T cell line by CBS monoclonal antibody (M01), clone 3E1.

CBS monoclonal antibody (M01), clone 3E1.

Western blot analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1).

Detection limit for recombinant GST tagged CBS is approximately 0.

ELISA

8 images

38 references

产品编号 ABIN7489109

50 μg

发货至: 中国

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详细

DYRK1A 抗体 (AA 674-763)

DYRK1A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7D10 unconjugated

DYRK1A monoclonal antibody (M01), clone 7D10.

Detection limit for recombinant GST tagged DYRK1A is approximately 0.

ELISA

Western Blot detection against Immunogen (35.

7 images

39 references

产品编号 ABIN560675

100 μg

发货至: 中国

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详细

AGO2 抗体 (AA 1-377)

AGO2 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 2E12-1C9 unconjugated

Western blot analysis of EIF2C2 over-expressed 293 cell line, cotransfected with EIF2C2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1).

Immunoperoxidase of monoclonal antibody to EIF2C2 on formalin-fixed paraffin-embedded human stomach.

ISt

Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell.

Detection limit for recombinant GST tagged EIF2C2 is 0.

ELISA

Western Blot detection against Immunogen (68.

Western Blot analysis of EIF2C2 expression in transfected 293T cell line by EIF2C2 monoclonal antibody (M01), clone 2E12-1C9.

EIF2C2 monoclonal antibody (M01), clone 2E12-1C9.

7 images

44 references

产品编号 ABIN565346

100 μg

发货至: 中国

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详细

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