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NeuN 抗体 (AA 1-140)

RBFOX3 适用: 人, 小鼠 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1H3B2 unconjugated
产品编号 ABIN5542704
发货至: 中国
  • 抗原 See all NeuN (RBFOX3) 抗体
    NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
    抗原表位
    • 16
    • 5
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    AA 1-140
    适用
    • 52
    • 43
    • 38
    • 16
    • 4
    • 3
    • 1
    • 1
    • 1
    人, 小鼠
    宿主
    • 42
    • 14
    • 2
    小鼠
    克隆类型
    • 32
    • 26
    单克隆
    标记
    • 27
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This NeuN antibody is un-conjugated
    应用范围
    • 36
    • 18
    • 18
    • 14
    • 14
    • 13
    • 11
    • 10
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)
    免疫原
    Purified recombinant fragment of human RBFOX3 (AA 1-140) expressed in E. Coli.
    克隆位点
    1H3B2
    亚型
    IgG2a
  • 应用备注
    ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: , ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
    限制
    仅限研究用
  • 生效 #104496 (Western Blotting)
    '独立验证'标志
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #104496
    日期
    2023.08.02
    抗原
    RBFOX3
    Lot Number
    160425
    Method validated
    Western Blotting
    Positive Control

    Adult mouse brain and cerebellum

    Negative Control
    Notes

    The NeuN antibody ABIN5542704 works in WB at a 1:1000 dilution with sensitive ECL substrate on adult mouse brain and cerebellum extracts. Non-specific bands are also detected.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN5542704
    Secondary Antibody

    HRP-conjugated goat anti-mouse IgG (Sigma-Aldrich, NA931VS)

    Full Protocol
    • Homogenize tissues with cold lysis buffer containing 50 mM Tris HCl, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, and 1% protease inhibitor (Sigma P8340) using an ultrasonic homogenizer (MSE, SoniPrep 150) with 16 amplitude, 20 s on, 10 s off pulse for 60 s.
    • Centrifuge tissue homogenates at 13,000 rpm for 20 min at 4 °C.
    • Collect supernatants and determine total protein content using a Bradford assay.
    • Denature 50 µg of total protein for 5 min at 90 °C and subsequently separate them on a denaturing 12% PAGE-SDS gel alongside a Precision Plus Protein Dual Color Standard (Bio-Rad, 160374).
    • Electro-transfer proteins onto nitrocellulose membrane (Amersham Biosciences, RPN203D) ON in the cold room.
    • Wash membrane 3x for 10 min with 0.01 M PBS containing 0.1% Tween-20 (PBST).
    • Block membrane with PBST containing 2% bovine serum albumin for 1 h at RT.
    • Incubate membrane with primary mouse anti-NeuN antibody (antibodies-online, ABIN5542704, lot 160425) diluted 1:1,000 in PBST ON at 4 °C.
    • Wash membrane 3x 10 min with PBST.
    • Incubate membrane with secondary HRP-conjugated goat anti-mouse IgG (Sigma-Aldrich, NA931VS) diluted 1:1,000 in PBST for 1 h at RT.
    • Wash membrane 3x 10 min with PBST.
    • Visualize proteins with WesternBright Sirius HRP substrate (Advansta) using a ChemiDoc Imaging System.
    Experimental Notes

    Non-specific bands were also detected in adult mouse cerebellum tissue extracts, but not on laser-microdissected Purkinje neurons.

  • 状态
    Liquid
    缓冲液
    PBS with 0.05 % sodium azide
    储存条件
    4 °C,-20 °C
    储存方法
    4°C, -20°C for long term storage
  • 抗原
    NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
    别名
    RBFOX3 (RBFOX3 产品)
    别名
    FOX-3 antibody, FOX3 antibody, HRNBP3 antibody, NEUN antibody, Fox-3 antibody, Hrnbp3 antibody, NeuN antibody, Neuna60 antibody, Neun antibody, RGD1560070 antibody, RNA binding fox-1 homolog 3 antibody, RNA binding protein, fox-1 homolog (C. elegans) 3 antibody, RNA binding protein, fox-1 homolog 3 antibody, RBFOX3 antibody, Rbfox3 antibody
    背景
    RBFOX3 (RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3) is a Protein Coding gene. Diseases associated with RBFOX3 include vertebral artery occlusion and tuberculum sellae meningioma. Among its related pathways are Neuroscience. GO annotations related to this gene include nucleic acid binding and nucleotide binding. An important paralog of this gene is RBFOX1.,
    分子量
    28.3kDa
    HGNC
    23492
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