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SYN1 抗体 (AA 362-511)

SYN1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, ICC, FACS, mIHC 宿主: 小鼠 Monoclonal 7H10G6 unconjugated
产品编号 ABIN5542390
发货至: 中国
  • 抗原 See all SYN1 抗体
    SYN1 (Synapsin I (SYN1))
    抗原表位
    • 27
    • 15
    • 15
    • 8
    • 7
    • 7
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 362-511
    适用
    • 116
    • 94
    • 75
    • 16
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    人, 小鼠, 大鼠, 猴
    宿主
    • 123
    • 6
    小鼠
    克隆类型
    • 115
    • 14
    单克隆
    标记
    • 75
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This SYN1 antibody is un-conjugated
    应用范围
    • 86
    • 43
    • 30
    • 20
    • 16
    • 13
    • 13
    • 7
    • 7
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Multiplex Immunohistochemistry (mIHC)
    纯化方法
    purified
    免疫原
    Purified recombinant fragment of human SYN1 (AA: 362-511) expressed in E. coli.
    克隆位点
    7H10G6
    亚型
    IgG1
  • 应用备注
    ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
    限制
    仅限研究用
  • 生效 #104335 (Multiplex Immunohistochemistry)
    '独立验证'标志
    by
    Akoya Biosciences
    No.
    #104335
    日期
    2021.04.20
    抗原
    SYN1
    Lot Number
    151229
    Method validated
    Multiplex Immunohistochemistry
    Positive Control

    FFPE normal human cortex

    Negative Control

    Unlabeled control (FFPE normal human cortex)

    Notes

    Passed. The anti-SYN1 antibody ABIN5542390 produces punctate labeling in synapse-rich regions of the human cortex. This pattern is consistent with expected SYN1 localization in synaptic contacts.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN5542390
    Secondary Antibody
    Full Protocol
    • Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
    • Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
    • Conjugation of the anti-SYN1 antibody ABIN5542390 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.
    Experimental Notes
    • No signal was detected in unlabeled specimens.

    • Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

    • Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

  • 状态
    Liquid
    缓冲液
    Purified antibody in PBS with 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    4°C, -20°C for long term storage
  • 抗原
    SYN1 (Synapsin I (SYN1))
    别名
    SYN1 (SYN1 产品)
    别名
    SYN1a antibody, SYN1b antibody, SYNI antibody, Syn-1 antibody, SYN I antibody, si:dkey-90n12.3 antibody, synapsin I antibody, synapsin I L homeolog antibody, SYN1 antibody, Syn1 antibody, syn1.L antibody, syn1 antibody
    背景

    Description: This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.,

    Aliases: SYNI, SYN1a, SYN1b

    分子量
    74.1 kDa
    基因ID
    6853
    HGNC
    6853
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