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CD45 抗体 (AA 535-730)

Name: CD45 抗体 (AA 535-730)
SKU: ABIN967867
Availability: OutOfStock

PTPRC 适用: 人, 小鼠, 大鼠 IHC, WB, IF, IP 宿主: 小鼠 Monoclonal 69-CD45 unconjugated

Western blot analysis of CD45 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). (CD45 抗体 (AA 535-730))

Immunofluorescence staining of Jurkat cells (Human T-cell leukemia, ATCC TIB-152). (CD45 抗体 (AA 535-730))

2 images

(5 references)

产品编号 ABIN967867

发货至: 中国

Datasheet as PDF

抗原 See all CD45 (PTPRC) 抗体

CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
抗原表位
16

16

14

13

12

10

9

7

6

6

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 535-730
适用
348

208

79

29

20

16

15

10

7

6

6

4

4

3

3

3

2

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
246

160

137

2

1

1
小鼠
Compatible Secondaries
克隆类型
411

128

1
单克隆
标记
165

52

44

43

33

13

12

11

10

9

8

8

8

6

6

6

6

6

6

5

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This CD45 antibody is un-conjugated
应用范围
445

187

147

120

91

58

43

39

38

27

15

14

13

12

9

5

4

3

3

2

2

1

1

1

1

1

1
Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

交叉反应

小鼠, 大鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human CD45 aa. 535-730 (alternatively spliced)

克隆位点

69-CD45

亚型

IgG1

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说明

Related Products: ABIN968537, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Wu, Fu, Shen: "SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription." in: Molecular and cellular biology, Vol. 22, Issue 8, pp. 2673-86, (2002) (PubMed).

Wang, Liang, Esselman: "Regulation of the calcium/NF-AT T cell activation pathway by the D2 domain of CD45." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 5, pp. 2557-64, (2000) (PubMed).

Phipps, Yousefi, Branch: "Increased enzymatic activity of the T-cell antigen receptor-associated fyn protein tyrosine kinase in asymptomatic patients infected with the human immunodeficiency virus." in: Blood, Vol. 90, Issue 9, pp. 3603-12, (1997) (PubMed).

Biffen, McMichael-Phillips, Larson, Venkitaraman, Alexander: "The CD45 tyrosine phosphatase regulates specific pools of antigen receptor-associated p59fyn and CD4-associated p56lck tyrosine in human T-cells." in: The EMBO journal, Vol. 13, Issue 8, pp. 1920-9, (1994) (PubMed).

Sieh, Bolen, Weiss: "CD45 specifically modulates binding of Lck to a phosphopeptide encompassing the negative regulatory tyrosine of Lck." in: The EMBO journal, Vol. 12, Issue 1, pp. 315-21, (1993) (PubMed).
抗原

CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

别名

CD45 (Ly-5) (PTPRC 产品)

别名

PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody

背景

CD45 (also known as leukocyte common antigen, GP180, or T200) is found on all hematopoietic cells except those of red cell origin. The N-terminal domain is a large glycosylated extracellular region of variable length (390-542 amino acids) derived by the alternative splicing of at least three exons (4, 5, or 6). This variation accounts for the different isoforms of CD45 (180-220 kDa). The remainder of the molecule includes a short transmembrane region followed by a large highly conserved cytoplasmic domain of 702 residues. The CD45 cytoplasmic domain possesses an intrinsic protein tyrosine phosphatase (PTPase) activity. This region contains two PTPase domains (domains I and II) which share 40% homology. Both domains are required for optimal PTPase activity and show significant homology with other receptor-like and cytoplasmic PTPases. CD45 plays a critical role in antigen receptor-induced responses of T and B cells. In B cells, CD45 can exist as part of a larger multisubunit complex composed of several tyrosine phosphorylated proteins, including the Src-family PTK, Lyn, or it can exist as the membrane immunoglobulin-associated MB-1/B29 heterodimer. In T cells, CD45 associates with and dephosphorylates both lck and fyn. In addition, CD45 can associate with membrane proteins involved in T cell activation, such as CD2, Thy-1, TCR, and CD26.
Synonyms: Leukocyte Common Antigen, GP180, T200

分子量

180-220 kDa

途径

TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling