CD45 抗体
(1 reference)-
- 抗原 See all CD45 (PTPRC) 抗体
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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适用
- 人, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD45 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Staining Methods (StM)
- 特异性
- Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180 kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Isolated neoplastic cells from T cell lymphoma (2B11), and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26)
- 克隆位点
- 2B11-PD7-26
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product PTPRC Primary Antibody
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anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 1210-1304) antibody
PTPRC 适用: 人, 小鼠, 大鼠, 兔 FACS, WB, IHC (p), ELISA, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 193-575) antibodyPTPRC 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 371-556) antibodyPTPRC 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 24-575) antibodyPTPRC 适用: 人 FACS, IHC, WB, IF, ELISA 宿主: 小鼠 Monoclonal 17H9D4 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (C-Term) antibodyPTPRC 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Cow, Cat, 兔, 绵羊, Pig, 豚鼠, 山羊, 马, 猴, Avian, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibodyPTPRC 适用: 人 FACS, IHC, WB, IF, StM 宿主: 兔 Monoclonal PTPRC-2877R unconjugated Recombinant Antibody
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibodyPTPRC 适用: 人 FACS, WB, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal MEM-28 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 378-577) antibodyPTPRC 适用: 人, 小鼠, 大鼠 FACS, IHC, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 2H3E2 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 431-577) antibodyPTPRC 适用: 人 FACS, IHC, WB, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 7C9F12 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 928-989) antibodyPTPRC 适用: 人 IHC, WB, ELISA 宿主: 小鼠 Monoclonal 5D3A3 unconjugated
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- 应用备注
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Positive Control: Jurkat, Ramos, U-698 or GA-10 cells. Tonsil and Spleen.
Known Application: Flow Cytometry (0.5-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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: "Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas." in: PloS one, Vol. 17, Issue 9, pp. e0274261, (2022) (PubMed).
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: "Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas." in: PloS one, Vol. 17, Issue 9, pp. e0274261, (2022) (PubMed).
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- 抗原
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- 别名
- PTPRC (PTPRC 产品)
- 别名
- PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody
- 分子量
- 180-220kDa
- 基因ID
- 5788
- UniProt
- P08575
- 途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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