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CD45 抗体 (AA 1210-1304)

Name: CD45 抗体 (AA 1210-1304)
SKU: ABIN669606
Availability: OutOfStock

PTPRC 适用: 人, 小鼠, 大鼠, 兔 FACS, WB, IHC (p), ELISA, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

Raji cells probed with CD45 Polyclonal Antibody, Unconjugated at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (CD45 抗体 (AA 1210-1304))

FACS

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-CD45/B220 Polyclonal Antibody, Unconjugated (ABIN669606) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD45 抗体 (AA 1210-1304))

IHC

Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled with Rabbit Anti-CD45/B220 Polyclonal Antibody, Unconjugated (ABIN669606) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD45 抗体 (AA 1210-1304))

IHC

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD45 Polyclonal Antibody, Unconjugated (ABIN669606) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C (CD45 抗体 (AA 1210-1304))

Lane 1: Mouse spleen lysates; Lane 2: Human raji lysates probed with Rabbit Anti-CD45 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (CD45 抗体 (AA 1210-1304))

Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled with Rabbit Anti-CD45/B220 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD45 抗体 (AA 1210-1304))

IHC (p)

6 images

(6 references)

产品编号 ABIN669606

发货至: 中国

Datasheet as PDF

抗原 See all CD45 (PTPRC) 抗体

CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
抗原表位
16

15

14

14

13

10

9

7

6

6

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1210-1304
适用
384

226

78

29

19

16

15

10

7

6

6

4

4

3

3

3

2

1

1

1

1

1

1
人, 小鼠, 大鼠, 兔
宿主
282

178

138

2

1

1
兔
Compatible Secondaries
克隆类型
467

127

1
多克隆
标记
174

58

49

46

38

15

13

11

10

10

9

9

8

8

7

6

6

6

6

6

5

5

5

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1
This CD45 antibody is un-conjugated
应用范围
495

197

148

127

126

74

58

57

47

26

17

15

14

12

12

5

4

3

3

3

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

特异性

The antibody is predicted to work with Mouse and Rat samples, but with decreased sensitivity compared to human samples.

交叉反应

人, 小鼠, 兔, 大鼠

预测反应

Cow

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human CD45

亚型

IgG
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody
PTPRC 适用: 人, 犬 FACS, IHC, WB, StM 宿主: 小鼠 Monoclonal 2B11-PD7-26 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 193-575) antibody
PTPRC 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 371-556) antibody
PTPRC 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 24-575) antibody
PTPRC 适用: 人 FACS, IHC, WB, IF, ELISA 宿主: 小鼠 Monoclonal 17H9D4 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (C-Term) antibody
PTPRC 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Cow, Cat, 兔, 绵羊, Pig, 豚鼠, 山羊, 马, 猴, Avian, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody
PTPRC 适用: 人 FACS, IHC, WB, IF, StM 宿主: 兔 Monoclonal PTPRC-2877R unconjugated Recombinant Antibody

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody
PTPRC 适用: 人 FACS, WB, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal MEM-28 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 378-577) antibody
PTPRC 适用: 人, 小鼠, 大鼠 FACS, IHC, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 2H3E2 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 431-577) antibody
PTPRC 适用: 人 FACS, IHC, WB, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 7C9F12 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 928-989) antibody
PTPRC 适用: 人 IHC, WB, ELISA 宿主: 小鼠 Monoclonal 5D3A3 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhu, Meng, Wang, Wang, Zhang, Li, Li, Tan, Yang, Huang: "Effects of neuritin on the differentiation of bone marrow‑derived mesenchymal stem cells into neuron‑like cells." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 3201-3207, (2018) (PubMed).

Liao, Zhong, Liu, Li, Ling, Zou: "Bone mesenchymal stem cells co-expressing VEGF and BMP-6 genes to combat avascular necrosis of the femoral head." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 954-962, (2018) (PubMed).

Tsang, Huang, Yang, Chi, Chiang, Wang, Andocs, Szasz, Li, Chi: "Improving immunological tumor microenvironment using electro-hyperthermia followed by dendritic cell immunotherapy." in: BMC cancer, Vol. 15, pp. 708, (2015) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Zeng, Hu, Yuan, Hu, Zhong, Kang: "Increased expression of importin13 in endometriosis and endometrial carcinoma." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 18, Issue 6, pp. CR361-7, (2012) (PubMed).
抗原

CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

别名

CD45 (PTPRC 产品)

别名

PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody

背景

Synonyms: LCA, LY5, B220, CD45, L-CA, T2, CD45R, GP18, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, PTPRC
Background: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).

基因ID

5788

UniProt

P08575

途径

TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling