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IRS1 抗体 (pTyr896)

Name: IRS1 抗体 (pTyr896)
SKU: ABIN967601
Availability: OutOfStock

This anti-IRS1 antibody is a 小鼠单克隆 antibody detecting IRS1 in WB. Suitable for 人. This Primary Antibody has been cited in 5+ publications.

Western blot analysis of IRS-1 (pY896) in transformed human epithelial cells. (IRS1 抗体 (pTyr896))

1 image

(5 references)

产品编号 ABIN967601

发货至: 中国

Datasheet as PDF

Quick Overview for IRS1 抗体 (pTyr896) (ABIN967601)

抗原

See all IRS1 抗体

IRS1 (Insulin Receptor Substrate 1 (IRS1))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This IRS1 antibody is un-conjugated

应用范围

Western Blotting (WB)

克隆位点

K9-211

Discover our top product IRS1 Primary Antibody

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pTyr896

品牌

BD Pharmingen™

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Phosphorylated Human IRS-1 Peptide

亚型

IgG2a kappa
anti-Insulin Receptor Substrate 1 (IRS1) (pSer639) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer312) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (AA 1041-1242) antibody
IRS1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer307) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (AA 106-122), (N-Term) antibody
IRS1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody
IRS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (Ser312) antibody
IRS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (AA 274-323), (pSer307) antibody
IRS1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer307) antibody
IRS1 适用: 人, 小鼠, 大鼠, 山羊 ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN967389

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Gual, Le Marchand-Brustel, Tanti: "Positive and negative regulation of insulin signaling through IRS-1 phosphorylation." in: Biochimie, Vol. 87, Issue 1, pp. 99-109, (2005) (PubMed).

White: "IRS proteins and the common path to diabetes." in: American journal of physiology. Endocrinology and metabolism, Vol. 283, Issue 3, pp. E413-22, (2002) (PubMed).

Burks, White: "IRS proteins and beta-cell function." in: Diabetes, Vol. 50 Suppl 1, pp. S140-5, (2001) (PubMed).

Paz, Liu, Shorer, Hemi, LeRoith, Quan, Kanety, Seger, Zick: "Phosphorylation of insulin receptor substrate-1 (IRS-1) by protein kinase B positively regulates IRS-1 function." in: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28816-22, (1999) (PubMed).

Ward, Gough, Rashke, Wan, Tribbick, Wang: "Systematic mapping of potential binding sites for Shc and Grb2 SH2 domains on insulin receptor substrate-1 and the receptors for insulin, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor." in: The Journal of biological chemistry, Vol. 271, Issue 10, pp. 5603-9, (1996) (PubMed).
抗原

IRS1 (Insulin Receptor Substrate 1 (IRS1))

别名

IRS-1

背景

The IRS (Insulin Receptor Substrate) proteins IRS-1, IRS-2, IRS-3, and IRS-4 are major substrates of the insulin receptor and the insulin-like growth factor-1 (IGF-1) receptor tyrosine kinases. IRS proteins contain an N-terminal pleckstrin homology (PH) domain, a phosphotyrosine-binding (PTB) domain, and multiple tyrosine phosphorylation sites in the C-terminus. The IRS-1 protein is widely expressed and, along with IRS-2, mediates somatic growth and carbohydrate metabolic responses to insulin. Following insulin receptor ligation, IRS-1 binds to the juxtamembrane region of the receptor via the PH and PTB domains and is tyrosine phosphorylated, which facilitates its interaction with SH2 domain-containing signaling proteins. Specifically, the phosphorylated tyrosine 896 (pY896) of human IRS-1 is a major binding site for the GRB2 (Growth-factor Receptor-Bound protein 2) adaptor protein. After IRS-1 activation, negative and positive feedback regulates dephosphorylation of its tyrosine sites, which ultimately regulates the magnitude and/or duration of the downstream pleiotropic responses to insulin and IGF-1.
The K9-211 monoclonal antibody recognizes pY896 of human IRS-1. The orthologous phosphorylation sites of mouse and rat IRS-1 are Y891 and Y895, respectively.

分子量

160-185 kDa

途径

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process