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IRS1 抗体 (N-Term)

Name: IRS1 抗体 (N-Term)
SKU: ABIN3042564
Availability: OutOfStock
Rating: 5 (1 reviews)

IRS1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Insulin Receptor Substrate 1 (IRS1) (AA 106-122), (N-Term) antibody (ABIN3042564)

IHC

Western Blotting (WB) image for anti-Insulin Receptor Substrate 1 (IRS1) (AA 106-122), (N-Term) antibody (ABIN3042564)

2 images

(1 reference)

(1 validation)

产品编号 ABIN3042564

发货至: 中国

Datasheet as PDF

抗原 See all IRS1 抗体

IRS1 (Insulin Receptor Substrate 1 (IRS1))
抗原表位
31

28

23

21

21

19

18

17

16

16

16

5

5

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 106-122, N-Term
适用
290

233

209

23

19

18

17

16

16

15

2

1

1
人
宿主
290

12
兔
Compatible Secondaries
克隆类型
284

18
多克隆
标记
136

14

12

12

12

12

12

12

11

10

10

10

10

10

10

9
This IRS1 antibody is un-conjugated
应用范围
170

126

104

93

92

67

58

51

24

20

13

7

4

2

2

1
Western Blotting (WB)

原理

Rabbit IgG polyclonal antibody for Insulin receptor substrate 1(IRS1) detection. Tested with WB in Human,Mouse,Rat.

序列

WYQALLQLHN RAKAHHD

交叉反应（详细）

Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

产品特性

Rabbit IgG polyclonal antibody for Insulin receptor substrate 1(IRS1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: insulin receptor substrate 1
Protein Name: Insulin receptor substrate 1

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of mouse IRS1(106-122aa WYQALLQLHNRAKAHHD), identical to the related rat sequence, and different from the related human sequence by one amino acid.

亚型

IgG

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anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer639) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer312) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (AA 1041-1242) antibody
IRS1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer307) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody
IRS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (Ser312) antibody
IRS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pSer312) antibody
IRS1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, DB 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (pTyr612) antibody
IRS1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, DB 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor Substrate 1 (IRS1) (AA 274-323), (pSer307) antibody
IRS1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

限制

仅限研究用
生效 #300033 (Immunohistochemistry)

by

Human Protein Atlas

No.

#300033

日期

2016.12.13

抗原

IRS1

Lot Number

Method validated

Immunohistochemistry

Positive Control

colon, tonsil

Negative Control

Notes

Passed. ABIN3042564 staining is consistent with RNA-seq data and published literature.

Validation Images

Staining of human colon (A.) and tonsil (B.) sections in a tissue microarray with ABIN3042564. See the protocol for detailed information.
Full Methods
Primary Antibody

ABIN3042564

Secondary Antibody

HRP polymer (ThermoFisher Scientific, TL-125-PH)

Full Protocol

Sample preparation:
Fixation and TMA preparation according to PMID: 22688270.
Cut 4µm TMA sections using a waterfall microtome (ThermoFisher Scientific, HM355S).
Dry sections ON at RT and then back them for 12-24h at 50°C.
Deparaffinization and rehydration in xylene and graded alcohol series:
xylene twice for 3min.
100% ethanol for 1min.
95% ethanol for 1min.
0.3% H₂O₂ in 95% ethanol for 5min to block endogenous peroxidase.
70% ethanol for 1min.
50% ethanol for 1min.
distilled H₂O for 2min.
Antigen Retrieval by Heat Induced Epitope Retrieval (HIER):
Boil the sections in Lab Vision Citrate buffer pH6.0 (ThermoFisher Scientific, TA-250-PM1X) for 4min at 125°C in a decloaking chamber (Biocare Medical).
Allow slides to cool down to 90°C in the decloaking chamber.
Immunostaining in a Lab Vision Autostainer 480 (ThermorFisher Scientific) at RT with 300µl of reagents for each step:
Rinse sections in Lab Vision wash buffer with extra tween added to a final concentration of 0.2% (ThermoFisher Scientific, TA-999-TT and TA-125-TW) wash buffer.
Block sections with Lab Vision Ultra V-Block (ThermoFisher Scientific, TA-125-UB) for 5min.
Rinse sections 2x in wash buffer.
Incubate sections with primary rabbit anti-IRS1 antibody (antibodies-online, ABIN3042564) diluted 1:1100 for 30min.
Rinse sections 3x in wash buffer.
Incubate sections with labeled HRP polymer (ThermoFisher Scientific, TL-125-PH) for 30min.
Rinse sections 2x in wash buffer.
Develop in DAB Quanto solution (ThermoFisher Scientific, TL-125-QHDX) for 5min.
Rinse sections in distilled H₂O.
Washing, counterstaining, and coverslipping in Autostainer XL (Leica Biosystems, ST5010) at RT:
Counterstaining in Mayer’s hematoxylin plus (HistoLab, 01820) for 7.5min.
Rinse sections in tap water for 5min.
Rinse sections in lithium carbonate water diluted 1:5 for 5min.
Dehydration in graded ethanol series and Neo-Clear (Merck Millipore, 109843) according to the manufacturer recommendations.
Automated mounting (Leica Biosystems, CV5030) of coverslip with Pertex (Histolab, 00871).
Microscopy:
Image acquisition on a Leica Aperio AT2 and Aperio Scanscope AT at 20x magnification.

Experimental Notes

ABIN3042564 shows nice IRS1 staining in endothelial cells in most tissues as is supported by both literature and RNA-seq data. At lower dilutions the antibody does also show some cytoplasmic staining in the tested tissues.
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Mi, Chen, Zheng, Li, Zhang, Mo, Yang: "MicroRNA-139-5p Suppresses 3T3-L1 Preadipocyte Differentiation Through Notch and IRS1/PI3K/Akt Insulin Signaling Pathways." in: Journal of cellular biochemistry, Vol. 116, Issue 7, pp. 1195-204, (2015) (PubMed).
抗原

IRS1 (Insulin Receptor Substrate 1 (IRS1))

别名

IRS1 (IRS1 产品)

别名

HIRS-1 antibody, G972R antibody, IRS-1 antibody, IRS1IRM antibody, irs1 antibody, irsu antibody, IRS1 antibody, irs-1 antibody, irs1-a antibody, insulin receptor substrate 1 antibody, insulin receptor substrate 2 antibody, insulin receptor substrate 1 S homeolog antibody, IRS1 antibody, Irs1 antibody, irs2 antibody, irs1.S antibody

背景

Insulin receptor substrate 1(IRS-1) is a signalling adapter protein that in humans is encoded by the IRS-1 gene. It is mapped to 2q36.3. This gene exhibited no intrinsic enzyme activity, and it can serve as a docking protein involved in binding and activating other signal transduction molecules after being phosphorylated on tyrosine by insulin receptor kinase. IRS1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1(IGF-1) receptors to intracellular pathways PI3K/Akt and Erk MAP kinase pathways. IRS1 also plays important biological function for both metabolic and mitogenic(growth promoting) pathways. In addition to those, IRS1 is a key regulator of PI3K within malignant cells.

Synonyms: HIRS 1 antibody|HIRS1 antibody|Insulin Receptor Substrate 1 antibody||IRS 1 antibody|IRS-1 antibody|IRS1 antibody|OTTHUMP00000164234 antibody

UniProt

P35569

途径

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process