SREBF2 抗体 (AA 833-1141)
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- 抗原 See all SREBF2 抗体
- SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))
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抗原表位
- AA 833-1141
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SREBF2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human SREBP-2 aa. 833-1141
- 克隆位点
- IgG-1C6
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product SREBF2 Primary Antibody
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anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 399-427) antibody
SREBF2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 75-164) antibodySREBF2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 1-220) antibodySREBF2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) antibodySREBF2 适用: 人, 大鼠, 小鼠, Pig WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 801-900) antibodySREBF2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 304-353) antibodySREBF2 适用: 人, 大鼠, 小鼠, Pig, 犬, Cow, 豚鼠, 马, 小鸡, Bat, Hamster, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 434-483) antibodySREBF2 适用: 人, 大鼠, 小鼠, Pig, 犬, Cow, 豚鼠, 马, 小鸡, Bat, Hamster, 猴, 兔 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 455-469) antibodySREBF2 适用: 人 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 399-427) antibodySREBF2 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB24513 unconjugated
anti-Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (AA 402-431), (Middle Region) antibodySREBF2 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- The IgG-1C6 antibody may be used for western blot analysis (2 µg/ml) and immunoprecipitation (2 µg antibody/300-500 µg of total protein lysate). U-937 human histiocytic lymphoma cells (ATCC CRL-1593) or HeLa human cervical carcinoma cells (ATCC CCL-2) are recommended as additional positive controls.
- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SREBPs) and SREBP cleavage-activating protein." in: The Journal of biological chemistry, Vol. 272, Issue 32, pp. 20213-21, (1997) (PubMed).
: "Hairpin orientation of sterol regulatory element-binding protein-2 in cell membranes as determined by protease protection." in: The Journal of biological chemistry, Vol. 270, Issue 49, pp. 29422-7, (1996) (PubMed).
: "Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis." in: The EMBO journal, Vol. 15, Issue 5, pp. 1012-20, (1996) (PubMed).
: "Purification of an interleukin-1 beta converting enzyme-related cysteine protease that cleaves sterol regulatory element-binding proteins between the leucine zipper and transmembrane domains." in: The Journal of biological chemistry, Vol. 270, Issue 30, pp. 18044-50, (1995) (PubMed).
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: "Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SREBPs) and SREBP cleavage-activating protein." in: The Journal of biological chemistry, Vol. 272, Issue 32, pp. 20213-21, (1997) (PubMed).
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- 抗原
- SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))
- 别名
- SREBP-2 (SREBF2 产品)
- 别名
- SREBF2 antibody, srebp2 antibody, SREBP2 antibody, zgc:158371 antibody, AI608257 antibody, SREBP-2 antibody, SREBP2gc antibody, bHLHd2 antibody, lop13 antibody, nuc antibody, Srebf2_retired antibody, sterol regulatory element binding transcription factor 2 antibody, sterol regulatory element binding factor 2 antibody, sterol regulatory element binding transcription factor 2 L homeolog antibody, SREBF2 antibody, srebf2 antibody, Srebf2 antibody, srebf2.L antibody
- 背景
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SREBP-1 and -2 (sterol-regulatory element binding proteins-1 and -2) are transcription factors which participate in the control of cholesterol homeostasis. SREBP proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol. Upon activation of SREBP-1 or -2, a ~480-500 amino acid, N-terminal cleavage fragment of these proteins enters the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis. Proteases which cleave SREBPs have been identified and include SCA (SREBP-cleavage activity), as well as caspase-3, a key regulator of apoptotic pathways. SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis, suggesting that SREBPs may play some role in apoptotic processes. However, sterol-regulated vs. apoptosis-associated cleavage of SREBP proteins appears to be independently regulated. On SDS-PAGE, sterol-regulated cleavage fragments of SREBP proteins migrate more slowly (i.e., higher molecular weight) than do staurosporin-induced fragments. In addition, staurosporin-induced SREBP cleavage products may appear as a doublet, with the upper band representing a phosphorylated form of SREBP. On SDS-PAGE, full length, precursor forms of SREBP-1 and -2 migrate at ~125 kD, while proteolytic cleavage fragments may be observed as a cluster of bands between 60 - 70 kDa. The IgG-1C6 antibody recognizes human SREBP-2. The antibody recognizes the C-terminal of human SREBP-2. A fusion protein containing C-terminal amino acids 833-1141 of human SREBP-2, was used as immunogen. The antibody recognizes both the 125 kDa precursor and the 60-70 kDa COOH-terminal cleaved forms of SREBP-2.
Synonyms: Sterol Regulatory Element-Binding Factor 1, SREBF1 - 途径
- Regulation of Lipid Metabolism by PPARalpha
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