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p53 抗体

TP53 适用: 人, 猴, Cow WB, IP, IHC (f), IHC (fro), ICS 宿主: 小鼠 Monoclonal DO unconjugated
产品编号 ABIN967458
发货至: 中国
  • 抗原 See all p53 (TP53) 抗体
    p53 (TP53) (Tumor Protein P53 (TP53))
    适用
    • 890
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    人, 猴, Cow
    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This p53 antibody is un-conjugated
    应用范围
    • 720
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    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS)
    品牌
    BD Pharmingen™
    交叉反应
    猴, 牛
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Human Recombinant p53 protein
    克隆位点
    DO
    亚型
    IgG2b
    Top Product
    Discover our top product TP53 Primary Antibody
  • 应用备注
    Positive control cell lines include SK-BR-3 breast carcinoma cells (ATCC HTB-30), A431 epidermal carcinoma cells (ATCC CRL-1555) and CEM human leukemia cells (ATCC CCL-119). MCF-7 breast carcinoma cells (ATCC HTB-22) are suggested as a negative control. Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach, or breast tissue.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C.
  • Baas, Mulder, Offerhaus, Vogelstein, Hamilton: "An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms." in: The Journal of pathology, Vol. 172, Issue 1, pp. 5-12, (1994) (PubMed).

    Cripps, Purdie, Carder, White, Komine, Bird, Wyllie: "A study of stabilisation of p53 protein versus point mutation in colorectal carcinoma." in: Oncogene, Vol. 9, Issue 9, pp. 2739-43, (1994) (PubMed).

    Jacquemier, Molès, Penault-Llorca, Adélaide, Torrente, Viens, Birnbaum, Theillet: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, (1994) (PubMed).

    Lambkin, Mothersill, Kelehan: "Variations in immunohistochemical detection of p53 protein overexpression in cervical carcinomas with different antibodies and methods of detection." in: The Journal of pathology, Vol. 172, Issue 1, pp. 13-8, (1994) (PubMed).

    Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, (1992) (PubMed).

    Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, (1991) (PubMed).

  • 抗原
    p53 (TP53) (Tumor Protein P53 (TP53))
    别名
    p53 (TP53 产品)
    别名
    BCC7 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, p53 antibody, Trp53 antibody, brp53 antibody, drp53 antibody, etID22686.5 antibody, fb40d06 antibody, wu:fb40d06 antibody, zgc:111919 antibody, Tp53 antibody, bbl antibody, bfy antibody, bhy antibody, p44 antibody, tp53 antibody, Xp53 antibody, TP53 antibody, tumor protein p53 antibody, cellular tumor antigen p53 antibody, transformation related protein 53 antibody, tumor protein p53 L homeolog antibody, TP53 antibody, CpipJ_CPIJ002758 antibody, Tp53 antibody, tp53 antibody, Trp53 antibody, tp53.L antibody
    背景
    P53 is a 53 kD nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. The DO-7 antibody recognizes human wildtype and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-7 recognizes an epitope between amino acids 1-45 of known forms of human p53. Human recombinant p53 protein was used as immunogen. Wildtype 53 proteins have a very short half-life and are usually not detectable with monoclonal antibodies (mAbs) in normal tissues.
    分子量
    53 kDa
    途径
    p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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