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p53 抗体 (AA 1-393)

Name: p53 抗体 (AA 1-393)
SKU: ABIN1386190
Availability: OutOfStock

TP53 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Role of P53 and MDM2 modifications for P53 function and stability. (p53 抗体 (AA 1-393))

Paraformaldehyde-fixed, paraffin embedded rat bladder tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-p53 (FL-393) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (p53 抗体 (AA 1-393))

IHC (p)

Expression of miR-211 and mRNA and protein expressions of SIRT1, Bcl-2, Bax, and p53 in lens tissues of mice(A) miR-211 expression and mRNA and protein expressions of SIRT1, Bcl-2, Bax, and p53 in mice lens, (B) strip chart of SIRT1, Bcl-2, Bax, and p53 proteins, (C) expressions of SIRT1, Bcl-2, Bax, and p53 proteins in mice lens, *, P<0. (p53 抗体 (AA 1-393))

The protein expressions of SIRT1, Bcl-2, Bax, and p53 in each group(A) The protein strip chart, (B) the relative protein expression in seven groups, *, P<0. (p53 抗体 (AA 1-393))

4 images

(4 references)

产品编号 ABIN1386190

发货至: 中国

Datasheet as PDF

抗原 See all p53 (TP53) 抗体

p53 (TP53) (Tumor Protein P53 (TP53))
抗原表位
49

39

39

38

32

28

24

24

23

22

20

20

19

18

18

17

16

16

14

13

9

8

8

8

8

8

7

7

7

7

7

7

6

6

5

5

5

5

5

4

4

4

3

3

3

3

3

3

3

3
AA 1-393
适用
851

259

214

109

44

34

31

18

10

8

8

6

4

3

3

2

2

2

2

2

1

1

1

1
人, 小鼠, 大鼠
宿主
646

219

6

2

1
兔
Compatible Secondaries
克隆类型
599

274

1
多克隆
标记
474

48

36

26

21

13

13

13

13

13

13

13

13

13

13

12

11

11

11

11

11

11

11

5

5

5

5

5

5

2

2

2

2

2

2

2

1

1

1

1

1

1
This p53 antibody is un-conjugated
应用范围
688

323

242

214

192

138

123

118

113

60

52

32

20

17

13

12

12

9

9

8

6

4

3

2

2

2

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Sheep,Pig,Horse

纯化方法

Purified by Protein A.

免疫原

Full length human p53 protein

亚型

IgG

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TP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (AA 1-100) antibody
TP53 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) antibody
KO Validated TP53 适用: 人 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (N-Term) antibody
TP53 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (AA 301-393) antibody
TP53 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (pSer33) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (AA 94-201) antibody
TP53 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2C3 unconjugated

anti-Tumor Protein P53 (TP53) (AA 94-201) antibody
TP53 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Tumor Protein P53 (TP53) (pSer20) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (acLys319) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Sola, Magrin, Pedrioli, Pinton, Salvadè, Papin, Paganetti: "Tau affects P53 function and cell fate during the DNA damage response." in: Communications biology, Vol. 3, Issue 1, pp. 245, (2021) (PubMed).

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Zeng, Feng, Lin, Ma, Liu: "Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targetingSIRT1gene in diabetic cataract mice." in: Bioscience reports, Vol. 37, Issue 4, (2017) (PubMed).

Qin, Xiong, Liu, Yao, Zhou, Hua, Wang: "Effects of irreversible electroporation on cervical cancer cell lines in vitro." in: Molecular medicine reports, Vol. 14, Issue 3, pp. 2187-93, (2016) (PubMed).
抗原

p53 (TP53) (Tumor Protein P53 (TP53))

别名

p53 (TP53 产品)

别名

BCC7 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, p53 antibody, Trp53 antibody, brp53 antibody, drp53 antibody, etID22686.5 antibody, fb40d06 antibody, wu:fb40d06 antibody, zgc:111919 antibody, Tp53 antibody, bbl antibody, bfy antibody, bhy antibody, p44 antibody, tp53 antibody, Xp53 antibody, TP53 antibody, tumor protein p53 antibody, cellular tumor antigen p53 antibody, transformation related protein 53 antibody, tumor protein p53 L homeolog antibody, TP53 antibody, CpipJ_CPIJ002758 antibody, Tp53 antibody, tp53 antibody, Trp53 antibody, tp53.L antibody

背景

Synonyms: LFS1, p53, p53 Cellular Tumor Antigen, p53 Tumor Suppressor, Phosphoprotein p53, TP53, Transformation related protein 53, TRP53, Tumor protein p53, Tumour Protein p53.
Background: p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

基因ID

7157

UniProt

P04637

途径

p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect