p53 抗体 (AA 14-289)
(5 references)-
- 抗原 See all p53 (TP53) 抗体
- p53 (TP53) (Tumor Protein P53 (TP53))
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抗原表位
- AA 14-289
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适用
- 人, 小鼠, 大鼠, 猴, Cow, Hamster, 小鸡
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This p53 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 交叉反应
- 小鼠, 大鼠, Hamster, 猴, 牛, 小鸡
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human p53 aa. 14-289
- 克隆位点
- PAb 240
- 亚型
- IgG1
- Top Product
- Discover our top product TP53 Primary Antibody
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anti-Tumor Protein P53 (TP53) (pSer392) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 1-100) antibodyTP53 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (N-Term) antibodyTP53 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyKO Validated TP53 适用: 人 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 301-393) antibodyTP53 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer33) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2C3 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Tumor Protein P53 (TP53) (pSer20) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (acLys319) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- SV40-transformed COS-7 monkey kidney cells (ATCC CRL-1651) or other SV40-transformed cell lines are suggested as positive controls.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, (1994) (PubMed).
: "Immunohistochemical analysis of p53 expression in malignant lymphomas." in: The American journal of pathology, Vol. 141, Issue 6, pp. 1343-8, (1993) (PubMed).
: "p53 mutations are not selected for in simian virus 40 T-antigen-induced tumors from transgenic mice." in: Journal of virology, Vol. 66, Issue 2, pp. 641-9, (1992) (PubMed).
: "Expression of p53 protein in infiltrating and in-situ breast carcinomas." in: The Journal of pathology, Vol. 165, Issue 3, pp. 203-11, (1992) (PubMed).
: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." in: Oncogene, Vol. 6, Issue 9, pp. 1699-703, (1991) (PubMed).
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: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, (1994) (PubMed).
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- 抗原
- p53 (TP53) (Tumor Protein P53 (TP53))
- 别名
- p53 (TP53 产品)
- 别名
- BCC7 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, p53 antibody, Trp53 antibody, brp53 antibody, drp53 antibody, etID22686.5 antibody, fb40d06 antibody, wu:fb40d06 antibody, zgc:111919 antibody, Tp53 antibody, bbl antibody, bfy antibody, bhy antibody, p44 antibody, tp53 antibody, Xp53 antibody, TP53 antibody, tumor protein p53 antibody, cellular tumor antigen p53 antibody, transformation related protein 53 antibody, tumor protein p53 L homeolog antibody, TP53 antibody, CpipJ_CPIJ002758 antibody, Tp53 antibody, tp53 antibody, Trp53 antibody, tp53.L antibody
- 背景
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P53 is a nuclear phosphoprotein which acts as a tumor suppressor by providing a cell cycle checkpoint for DNA damage during S-phase. Mutations in wildtype p53 can indirectly alter the DNA binding and transcription factor activity of p53. By altering expression of genes normally regulated by p53, these mutations can result in both a loss of tumor suppressor function and a gain of oncogenic function. The majority of mutations in the p53 gene are missense mutations which alter the identity of an amino acid. These mutations may alter the conformation and thus increase the stability of the mutant p53 protein. p53 is expressed in all vertebrate species examined. p53 may be overexpressed in transformed cell lines, where it forms complexes with viral oncogenes including SV40 large T antigen and the adenovirus protein, E1B. p53 migrates at ~53 kDa on SDS-PAGE.
PAb 240 reacts with a conformational epitope between amino acids 156 and 214 of native p53. As such, Pab 240 recognizes only certain mutant forms of p53, as determined by immunoprecipitation. It detects both mutant and wildtype p53 in western blot analysis and immunohistochemistry of frozen tissue sections. It is thought that p53 mutations exert a common conformational change which results in expression of the Pab 240-specific epitope on mutant p53 molecules. A recombinant fusion protein of p53 sequence including amino acids 14-289 was used as immunogen. - 分子量
- 53 kDa
- 途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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