Nestin 抗体
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北京 101111
Quick Overview for Nestin 抗体 (ABIN953652)
抗原
See all Nestin (NES) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Nestin. Note that the sequences of the Mouse and Human Nestin genes are divergent, and it is unlikely that antibodies raised against the Mouse sequence would recognize Human Nestin.
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纯化方法
- Affinity Chromatography using a peptide column.
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免疫原
- Three synthetic peptides KLH conjugated corresponding to different regions of the Mouse Nestin gene product (NP_057910), but are shared with the Rat (NM_012987) protein sequence. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
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亚型
- Ig Fraction
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anti-Nestin (NES) (AA 178-399) antibody
NES 适用: 人 IHC, WB, ICC, IP 宿主: 小鼠 Monoclonal D10 unconjugated
anti-Nestin (NES) antibodyNES 适用: 人 IHC, WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 2C2F7 unconjugated
anti-Nestin (NES) (AA 252-290), (N-Term) antibodyNES 适用: 人, 小鼠, 大鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB16253 unconjugated
anti-Nestin (NES) antibodyNES 适用: 小鼠 IHC, ICC 宿主: 大鼠 Monoclonal 7A3 unconjugated
anti-Nestin (NES) (AA 698-844) antibodyNES 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal NES-2911 unconjugated
anti-Nestin (NES) (AA 1484-1500) antibodyNES 适用: 人, 小鼠 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 681-750) antibodyNES 适用: 人, 小鼠, 大鼠 ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1392-1621) antibodyNES 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.3 mg/mL
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缓冲液
- 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted in the dark at 2-8 °C.
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- Nestin (NES)
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别名
- Nestin
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背景
- Mouse Nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.Synonyms: NES, Nbla00170
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基因ID
- 18008
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NCBI登录号
- NP_057910
抗原
-
