Nestin 抗体 (AA 698-844)
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- 抗原 See all Nestin (NES) 抗体
- Nestin (NES)
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抗原表位
- AA 698-844
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Nestin antibody is un-conjugated
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应用范围
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or -internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.
- 交叉反应 （详细）
- Human,
- 纯化方法
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
- 免疫原
- Recombinant human NES protein fragment around aa 698-844 (exact sequence is proprietary)
- 克隆位点
- NES-2911
- 亚型
- IgG
- Top Product
- Discover our top product NES Primary Antibody
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anti-Nestin (NES) (AA 681-750) antibody
NES 适用: 人, 小鼠, 大鼠 ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 178-399) antibodyNES 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D4 unconjugated
anti-Nestin (NES) antibodyNES 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 小鼠 Monoclonal 2C2F7 unconjugated
anti-Nestin (NES) (AA 1484-1500) antibodyNES 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 252-290), (N-Term) antibodyNES 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB16253 unconjugated
anti-Nestin (NES) antibodyNES 适用: 小鼠 IHC, ICC 宿主: 大鼠 Monoclonal 7A3 unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1392-1621) antibodyNES 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: Human kidney, ovary, cervix, breast, colon, endometrium, cerebellum, lung, prostate, skin and human melanoma tissue. HeLa cell lysate. SH-SY5Y cells.
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
- 有效期
- 24 months
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- 抗原
- Nestin (NES)
- 别名
- NES (NES 产品)
- 别名
- paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody
- 背景
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ESTM 46, FLJ21841, Intermediate filament protein, Nbla00170, nes,Nestin (Cancer Stem Cell Marker)
Cellular localisation: Cytoplasm and membrane. - 分子量
- 190-200kDa
- 基因ID
- 10763, 527971
- UniProt
- P48681
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