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Nestin 抗体

Name: Nestin 抗体
Brand: antibodies-online
SKU: ABIN7161178
Availability: OutOfStock

NES 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 小鼠 Monoclonal 2C2F7 unconjugated

Western Blot Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate All lanes: NES antibody at 3 μg/mL Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 178 kDa Observed band size: 260 kDa

Overlay histogram showing Hela cells stained with ABIN7161178 (red line).

FACS

Immunohistochemistry of paraffin-embedded human tonsil tissue using ABIN7161178 at dilution of 1:100

IHC

Immunohistochemistry of paraffin-embedded human kidney tissue using ABIN7161178 at dilution of 1:100

IHC

Immunofluorescent analysis of Hela cells using ABIN7161178 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of PC-3 cells using ABIN7161178 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of U251 cells using ABIN7161178 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemistry of paraffin-embedded human melanoma using ABIN7161178 at dilution of 1:100

IHC

Overlay histogram showing U251 cells stained with ABIN7161178 (red line).

FACS

9 images

产品编号 ABIN7161178

发货至: 中国

Datasheet as PDF

抗原 See all Nestin (NES) 抗体

Nestin (NES)
适用
126

68

47

4

3

2

2

2

1

1

1

1
人
宿主
101

33

4

2

1
小鼠
Compatible Secondaries
克隆类型
106

35
单克隆
标记
61

10

9

8

7

4

4

4

4

4

4

4

3

3

3

3

3

1

1

1
This Nestin antibody is un-conjugated
应用范围
76

47

46

45

40

39

37

29

25

15

7

6

2

2

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

交叉反应

人

纯化方法

Protein G purified

免疫原

Recombinant Human Nestin protein

克隆位点

2C2F7

亚型

IgG1, IgG1 kappa

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NES 适用: 人, 小鼠, 大鼠 ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nestin (NES) (AA 178-399) antibody
NES 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D4 unconjugated

anti-Nestin (NES) (AA 1484-1500) antibody
NES 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Nestin (NES) (AA 252-290), (N-Term) antibody
NES 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Nestin (NES) (AA 1389-1416) antibody
NES 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB16253 unconjugated

anti-Nestin (NES) antibody
NES 适用: 小鼠 IHC, ICC 宿主: 大鼠 Monoclonal 7A3 unconjugated

anti-Nestin (NES) (AA 698-844) antibody
NES 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal NES-2911 unconjugated

anti-Nestin (NES) (AA 1389-1416) antibody
NES 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated

anti-Nestin (NES) antibody (FITC)
NES 适用: 人 IF, ICC 宿主: 小鼠 Monoclonal 10C2 FITC

anti-Nestin (NES) (AA 1392-1621) antibody
NES 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

Nestin (NES)

别名

NES (NES 产品)

别名

paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

背景

Background: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Aliases: Nestin, NES, Nbla00170

UniProt

P48681