我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

HLAG 抗体 (C-Term) (Biotin)

Name: HLAG 抗体 (C-Term) (Biotin)
Brand: antibodies-online
SKU: ABIN94381
Availability: OutOfStock

HLAG 适用: 人 WB, ELISA, FACS, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal 5A6G7 Biotin

Western Blotting analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). (HLAG 抗体 (C-Term) (Biotin))

Flow cytometry analysis (intracellular staining) of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC. (HLAG 抗体 (C-Term) (Biotin))

FACS

2 images

(5 references)

产品编号 ABIN94381

发货至: 中国

Datasheet as PDF

抗原 See all HLAG 抗体

HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
抗原表位
17

15

8

6

6

5

5

4

2

2

2

2

1

1

1

1

1

1

1

1

1
C-Term
适用
120

7

4

1

1

1

1

1
人
宿主
65

61

3

1

1
小鼠
Compatible Secondaries
克隆类型
69

62
单克隆
标记
50

20

18

14

8

6

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This HLAG antibody is conjugated to Biotin
应用范围
84

66

58

43

29

27

14

13

13

10

6

6

6

5

2

1

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

特异性

The mouse monoclonal antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

免疫原

C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.

克隆位点

5A6G7

亚型

IgG1

Top Product

Discover our top product HLAG Primary Antibody
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody
HLAG 适用: 人 WB, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-G-1 unconjugated

anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (PE)
HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 PE

anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody
HLAG 适用: 人 ELISA, FACS, IHC (fro), IP, ICC 宿主: 小鼠 Monoclonal MEM-G-9 unconjugated

anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody
HLAG 适用: 人 ELISA, FACS, IHC (fro), IP, ICC 宿主: 小鼠 Monoclonal MEM-G-9 unconjugated

anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)
HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal 87G FITC

anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)
HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 FITC

anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)
HLAG 适用: 人 ELISA, FACS, IHC (fro), IP, ICC 宿主: 小鼠 Monoclonal MEM-G-9 Biotin

anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (APC)
HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 APC

anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (PE)
HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal 87G PE

anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 62-89) antibody
HLAG 适用: 人 WB, ELISA, FACS, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

说明

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Poláková, Železníková, Russ: "HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, (2013) (PubMed).

Lafon, Prehaud, Megret, Lafage, Mouillot, Roa, Moreau, Rouas-Freiss, Carosella: "Modulation of HLA-G expression in human neural cells after neurotropic viral infections." in: Journal of virology, Vol. 79, Issue 24, pp. 15226-37, (2005) (PubMed).

Le Rond, Gonzalez, Gonzalez, Carosella, Rouas-Freiss: "Indoleamine 2,3 dioxygenase and human leucocyte antigen-G inhibit the T-cell alloproliferative response through two independent pathways." in: Immunology, Vol. 116, Issue 3, pp. 297-307, (2005) (PubMed).

Rebmann, Lemaoult, Rouas-Freiss, Carosella, Grosse-Wilde: "Report of the Wet Workshop for Quantification of Soluble HLA-G in Essen, 2004." in: Human immunology, Vol. 66, Issue 8, pp. 853-63, (2005) (PubMed).

Le Rond, Le Maoult, Créput, Menier, Deschamps, Le Friec, Amiot, Durrbach, Dausset, Carosella, Rouas-Freiss: "Alloreactive CD4+ and CD8+ T cells express the immunotolerant HLA-G molecule in mixed lymphocyte reactions: in vivo implications in transplanted patients." in: European journal of immunology, Vol. 34, Issue 3, pp. 649-60, (2004) (PubMed).
抗原

HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

别名

HLA-G (HLAG 产品)

别名

MHC-G antibody, B-F antibody, B-F-S04 antibody, B-F-S05 antibody, B-F-S06 antibody, B-F-S07 antibody, B-FI antibody, B-FIV antibody, BF2 antibody, BFa2 antibody, BFw-03 antibody, BFw-05 antibody, BFz-01 antibody, major histocompatibility complex, class I, G antibody, MHC BF1 class I antibody, HLA-G antibody, BF1 antibody

背景

Major histocompatibility complex, class I, G,Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.

基因ID

3135

UniProt

P17693

途径

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints